SNX25 antibody (AA 48-584)

Catalog No. ABIN7875212

This Rabbit Polyclonal antibody specifically detects SNX25 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SNX25 antibody (AA 48-584) (ABIN7875212)

Target: SNX25 (Sorting Nexin 25 (SNX25))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNX25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SNX25 Antibody

Binding Specificity: AA 48-584

Purpose: SNX25 Antibody / Sorting nexin 25

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SNX25 recombinant protein (Position: H48-K584) was used as the immunogen for the SNX25 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SNX25 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SNX25 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SNX25

Target: SNX25 (Sorting Nexin 25 (SNX25))

Alternative Name: SNX25

Background: SNX25 antibody detects Sorting nexin-25, a member of the sorting nexin family involved in endosomal trafficking, receptor recycling, and membrane remodeling. SNX25 plays critical roles in signal transduction and protein turnover by directing cargo sorting within endosomes. The SNX25 antibody is widely used in cell biology, immunology, and receptor signaling studies to explore intracellular trafficking and degradation pathways.

SNX25 is encoded by the SNX25 gene located on human chromosome 4q24. The protein is approximately 580 amino acids long and contains a conserved phox homology (PX) domain that binds phosphatidylinositol-3-phosphate and a Bin/Amphiphysin/Rvs (BAR) domain that senses and induces membrane curvature. These structural domains enable SNX25 to participate in tubular endosome formation and cargo retrieval to the Golgi or plasma membrane.

The SNX25 antibody detects a 67 kilodalton band by western blot and shows cytoplasmic and vesicular staining by immunofluorescence. SNX25 interacts with transforming growth factor beta receptor complexes and promotes their endocytic degradation, acting as a negative regulator of transforming growth factor beta signaling. By controlling receptor turnover, SNX25 influences cellular proliferation, differentiation, and immune tolerance.

SNX25 also regulates Toll-like receptor trafficking in macrophages, affecting cytokine production and innate immune responses. In neurons, SNX25 participates in the endosomal sorting of synaptic receptors and contributes to synaptic plasticity. Its expression is dynamically regulated by inflammatory cues and cellular stress, linking endosomal remodeling to signaling adaptation.

Aberrant expression of SNX25 has been associated with cancer progression and immune dysregulation. Downregulation enhances transforming growth factor beta activity, promoting fibrosis and epithelial-mesenchymal transition, while overexpression suppresses pro-inflammatory signaling. NSJ Bioreagents provides a validated SNX25 antibody optimized for western blot and flow cytometry, supporting detailed studies of receptor sorting, endosomal dynamics, and signaling modulation.

UniProt: Q9H3E2

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling