KIF3A antibody (AA 486-701)

Catalog No. ABIN7875231

The Rabbit Polyclonal anti-KIF3A antibody is suitable to detect KIF3A in samples from Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for KIF3A antibody (AA 486-701) (ABIN7875231)

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF3A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-KIF3A Antibody

Binding Specificity: AA 486-701

Purpose: Zebrafish Kif3a Antibody

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Kif3a recombinant protein (amino acids D486-Q701) was used as the immunogen for the Zebrafish Kif3a antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Kif3a antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Kif3a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for KIF3A

Target: KIF3A (Kinesin Family Member 3A (KIF3A))

Alternative Name: Kif3a

Background: Kinesin-like protein KIF3A is a protein that in humans is encoded by the KIF3A gene. KIF3A is one subunit of the heterotrimeric motor protein, kinesin-2, that was initially isolated from sea urchin egg/embryo cytosol using microtubule affinity purification. This motor consists of two kinesin-related subunits (called KIF3A and KIF3B or 3C in vertebrates) and an associated protein (KAP3), and it transports protein complexes, nucleic acids and organelles towards the plus ends of microtubule tracks within cells. Work done in a broad range of eukaryotic cells has revealed that heterotrimeric kinesin-2 is the primary motor protein driving the intra-flagellar transport of tubulins and other axonemal building blocks from the base of the ciliary/flagellar axoneme to their site of assembly at the distal tips. This process is required for cilium assembly/maintenance and cilium-based signalling which play key roles in various cell and developmental processes. For example, in vertebrate embryos, kinesin-2 function is required for cilia-dependent nodal flow and the development of left-right asymmetry.

UniProt: E9QB71

Pathways: Hedgehog Signaling