NPEPPS antibody (AA 487-851)

Catalog No. ABIN7875233

This Rabbit Polyclonal antibody specifically detects NPEPPS in IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for NPEPPS antibody (AA 487-851) (ABIN7875233)

Target: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPEPPS antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NPEPPS Antibody

Binding Specificity: AA 487-851

Purpose: Zebrafish Npepps Antibody / Aminopeptidase

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Npepps recombinant protein (amino acids R487-A851) was used as the immunogen for the Zebrafish Npepps antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Npepps antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Npepps antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NPEPPS

Target: NPEPPS (Aminopeptidase Puromycin Sensitive (NPEPPS))

Alternative Name: Npepps

Background: Npepps, or puromycin sensitive aminopeptidase, is a cytosolic metallopeptidase that plays an important role in protein turnover, peptide trimming, and the regulation of cellular responses to stress and inflammation. It belongs to the M1 family of aminopeptidases and functions by cleaving amino acids from the N terminus of peptides and small proteins.

In zebrafish, Npepps is broadly expressed during development and in adult tissues, with particularly strong expression in the brain, liver, and kidneys. Zebrafish Npepps is an ortholog of the human NPEPPS gene, and both proteins share conserved structural domains and enzymatic functions. The conservation between zebrafish and human Npepps supports its use as a model for studying the role of aminopeptidases in vertebrate biology.

Functionally, Npepps is involved in a variety of cellular processes, including neuropeptide metabolism, regulation of oxidative stress responses, and antigen presentation. It has also been implicated in the degradation of misfolded proteins and the clearance of damaged peptides, especially in the context of neurodegeneration and immune signaling. In zebrafish, knockdown or mutation of npepps can affect normal development, particularly in the central nervous system.

Because of its high degree of evolutionary conservation and functional importance in proteostasis, zebrafish Npepps is frequently studied in models of neurodevelopment, protein metabolism, and cellular stress. It is also used to investigate mechanisms of protein degradation that are relevant to human conditions such as Alzheimer disease, inflammation, and cancer.