SSH1 antibody (AA 489-921)

Catalog No. ABIN7875236

This Rabbit Polyclonal antibody specifically detects SSH1 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for SSH1 antibody (AA 489-921) (ABIN7875236)

Target: SSH1 (Slingshot Homolog 1 (SSH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SSH1 Antibody

Binding Specificity: AA 489-921

Purpose: SSH1 Antibody / Slingshot protein phosphatase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SSH1 recombinant protein (Position: E489-Y921) was used as the immunogen for the SSH1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SSH1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SSH1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SSH1

Target: SSH1 (Slingshot Homolog 1 (SSH1))

Alternative Name: SSH1

Background: SSH1 antibody detects Slingshot protein phosphatase 1, a serine/threonine phosphatase that regulates actin filament dynamics by dephosphorylating cofilin. The UniProt recommended name is Slingshot protein phosphatase 1 (SSH1). This cytoplasmic enzyme is crucial for actin cytoskeleton remodeling during cell motility, adhesion, and morphogenesis.

Functionally, SSH1 antibody identifies a 1,047-amino-acid phosphatase that activates cofilin by removing inhibitory phosphate groups at serine 3. SSH1 operates downstream of Rho family GTPases and is controlled by 14-3-3 proteins, oxidative signals, and phosphorylation. It promotes lamellipodia formation and cytoskeletal reorganization during migration and neuronal outgrowth.

The SSH1 gene is located on chromosome 12q24.13 and is expressed in brain, muscle, and epithelial tissues. SSH1 integrates signals from LIM kinase and Rac/Cdc42 pathways to coordinate actin turnover and filament severing. Through cofilin activation, SSH1 drives rapid actin recycling necessary for directional movement and cell shape maintenance.

Pathologically, altered SSH1 activity contributes to cancer metastasis, neurological disorders, and cardiac hypertrophy. Dysregulated actin remodeling via SSH1-cofilin imbalance leads to defective migration and cytoskeletal instability. Research using SSH1 antibody supports studies in cytoskeletal dynamics, phosphatase signaling, and cell motility.

SSH1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect actin-regulating phosphatases. NSJ Bioreagents provides SSH1 antibody reagents optimized for studies in cell migration, cytoskeletal remodeling, and signal transduction.

Structurally, Slingshot protein phosphatase 1 contains an N-terminal cofilin-binding region and a catalytic domain related to the dual-specificity phosphatase family. This antibody aids in analyzing SSH1's control over actin filament turnover and cellular movement.

UniProt: Q8WYL5

Pathways: CXCR4-mediated Signaling Events