ORAI1 antibody (AA 49-301)

Catalog No. ABIN7875254

This Rabbit Polyclonal antibody specifically detects ORAI1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ORAI1 antibody (AA 49-301) (ABIN7875254)

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ORAI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ORAI1 Antibody

Binding Specificity: AA 49-301

Purpose: Orai1 Antibody

Purification: Affinity purified

Immunogen: A human recombinant protein (amino acids A49-A301) was used as the immunogen for the Orai1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Orai1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Orai1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ORAI1

Target: ORAI1 (ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1))

Alternative Name: Orai1

Background: ORAI1 (ORAI calcium release-activated calcium modulator 1), also known as CRACM1, Calcium release-activated calcium channel protein 1 and TMEM142A, is a calcium selectiveion channel that in humans is encoded by the ORAI1 gene. Orai1 channels play an important role in the activation of T-lymphocytes. The loss of function mutation of Orai1 causes severe combined immunodeficiency(SCID) in humans. The mammalian orai family has two additional homologs, orai2 and orai3. Orai proteins share no homology with any other ion channel family of any other known proteins. They have 4 transmembrane domains and form tetramers. Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments. Glu106-to-asp (E106D) and glu190-to-gln (E190Q) substitutions in transmembrane helices 1 and 3, respectively, diminished calcium ion influx, increased current carried by monovalent cations, and rendered the channel permeable to cesium ion.Prakriya et al. showed that ORAI1 is a PM protein, and that CRAC channel function is sensitive to mutation of 2 conserved acidic residues in the transmembrane segments.

UniProt: Q96D31

Pathways: TCR Signaling, BCR Signaling