ADH6 antibody (AA 50-367)

Catalog No. ABIN7875406

The Rabbit Polyclonal anti-ADH6 antibody (ABIN7875406) specifically detects ADH6 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ADH6 antibody (AA 50-367) (ABIN7875406)

Target: ADH6 (Alcohol Dehydrogenase 6 (Class V) (ADH6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ADH6 Antibody

Binding Specificity: AA 50-367

Purpose: ADH6 Antibody / Alcohol dehydrogenase 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ADH6 recombinant protein (Position: E50-K367) was used as the immunogen for the ADH6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ADH6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ADH6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ADH6

Target: ADH6 (Alcohol Dehydrogenase 6 (Class V) (ADH6))

Alternative Name: ADH6

Background: ADH6 antibody detects Alcohol dehydrogenase class V (also known as ADH6), a zinc-dependent enzyme that catalyzes the oxidation of alcohols to aldehydes as part of ethanol and retinoid metabolism. Encoded by the ADH6 gene on chromosome 4q23, this enzyme belongs to the alcohol dehydrogenase family of oxidoreductases that use NAD+ as a cofactor. ADH6 is mainly expressed in liver tissue and contributes to the metabolism of short-chain alcohols and other substrates, complementing the activities of ADH1 and ADH4 isoenzymes.

Structurally, ADH6 is a homodimer of approximately 40 kilodalton subunits, each containing a zinc-binding catalytic domain. The enzyme exhibits broad substrate specificity, oxidizing both ethanol and retinol intermediates. While its catalytic efficiency toward ethanol is lower than ADH1A and ADH1B, ADH6 plays a significant role in the oxidation of aromatic and aliphatic alcohols, making it important for detoxification and vitamin A metabolism. Genetic variations in ADH6 influence alcohol metabolism rates and susceptibility to alcohol-related diseases.

The ADH6 antibody is used in enzymology, metabolism, and toxicology research to study alcohol oxidation and hepatic metabolism. Western blot analysis identifies a 40 kilodalton band corresponding to ADH6, while immunohistochemistry shows strong cytoplasmic staining in hepatocytes. This antibody supports investigation into liver enzyme expression profiles, ethanol metabolism, and the biochemical pathways linking alcohol consumption to liver injury.

Beyond hepatic metabolism, ADH6 participates in retinoid oxidation, converting retinol to retinal, a precursor of retinoic acid involved in gene regulation and differentiation. Dysregulation of ADH6 activity may impact vitamin A homeostasis and oxidative stress responses. NSJ Bioreagents provides the ADH6 antibody validated for its applications ensuring reliable detection of Alcohol dehydrogenase class V in metabolic and toxicological studies.

UniProt: P28332