HBP1 antibody (AA 51-506)

Catalog No. ABIN7875522

The Rabbit Polyclonal anti-HBP1 antibody is suitable to detect HBP1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for HBP1 antibody (AA 51-506) (ABIN7875522)

Target: HBP1 (HMG Box-Containing Protein 1 (HBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HBP1 Antibody

Binding Specificity: AA 51-506

Purpose: HBP1 Antibody / HMG-box transcription factor 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HBP1 recombinant protein (Position: D51-R506) was used as the immunogen for the HBP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HBP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HBP1

Target: HBP1 (HMG Box-Containing Protein 1 (HBP1))

Alternative Name: HBP1

Background: HBP1 antibody detects HMG-box transcription factor 1, a transcriptional repressor that modulates cell cycle progression, differentiation, and Wnt signaling. Encoded by the HBP1 gene on chromosome 7q22.3, this protein belongs to the high mobility group (HMG) family of DNA-binding transcription factors. HBP1 acts as a nuclear regulator that represses proliferation-associated genes, integrating signals from growth factors, oncogenes, and stress pathways to maintain cellular homeostasis and differentiation potential.

Structurally, HBP1 is a 513-amino-acid nuclear protein of approximately 60 kilodaltons containing an HMG-box DNA-binding domain and transcriptional repression domains that interact with histone deacetylases (HDACs) and chromatin remodeling complexes. These interactions enable HBP1 to repress genes such as CCND1 (Cyclin D1) and N-MYC, thereby inhibiting uncontrolled cell proliferation. HBP1 also contains regulatory phosphorylation sites that control nuclear localization and DNA-binding affinity in response to extracellular cues.

The HBP1 antibody is widely used in cell cycle, cancer, and transcription research to study gene repression, differentiation, and tumor suppression mechanisms. Western blot analysis detects a 60 kilodalton band corresponding to HBP1, while immunofluorescence shows strong nuclear localization in epithelial and neuronal cells. This antibody enables detailed examination of transcriptional networks controlling growth arrest and lineage commitment.

Functionally, HBP1 suppresses oncogenic transformation by inhibiting Wnt/beta-catenin signaling and antagonizing MYC-dependent transcription. It also promotes senescence and apoptosis in response to DNA damage and oxidative stress, acting as a barrier to tumorigenesis. Reduced HBP1 expression or mutation is associated with breast, lung, and colon cancers, where loss of repression contributes to hyperproliferation. Beyond cancer, HBP1 regulates differentiation programs in neurons, adipocytes, and muscle cells by coordinating chromatin remodeling and transcriptional silencing. The HBP1 antibody provides a valuable tool for exploring these pathways and assessing transcriptional regulation in normal and diseased tissues. NSJ Bioreagents validates this antibody for its applications, ensuring specificity and consistency in transcriptional research applications.

UniProt: O60381