ITGA9 antibody (AA 526-1035)

Catalog No. ABIN7875584

This Rabbit Polyclonal antibody specifically detects ITGA9 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for ITGA9 antibody (AA 526-1035) (ABIN7875584)

Target: ITGA9 (Integrin alpha-9 (ITGA9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ITGA9 Antibody

Binding Specificity: AA 526-1035

Purpose: ITGA9 Antibody / Integrin alpha 9

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Integrin alpha-9/ITGA9 recombinant protein (Position: Q526-Q1035) was used as the immunogen for the ITGA9 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ITGA9 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ITGA9 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ITGA9

Target: ITGA9 (Integrin alpha-9 (ITGA9))

Alternative Name: ITGA9

Background: ITGA9 antibody detects Integrin alpha-9, a transmembrane receptor that mediates cell adhesion, migration, and signal transduction by binding to extracellular matrix (ECM) ligands such as tenascin-C, osteopontin, and VCAM1. Integrins are heterodimeric proteins composed of alpha and beta subunits, Integrin alpha-9 pairs with the beta-1 subunit to form the alpha9beta1 integrin complex. This receptor is involved in developmental morphogenesis, angiogenesis, epithelial integrity, and immune cell trafficking. The alpha9beta1 integrin recognizes diverse ligands containing the LDV or SVVYGLR motifs and promotes cytoskeletal reorganization and cell movement through activation of focal adhesion kinase and Rho-family GTPases.

Integrin alpha-9 is encoded by the ITGA9 gene located on chromosome 3p22.2. It is expressed in smooth muscle cells, fibroblasts, airway epithelium, and leukocytes. During embryonic development, Integrin alpha-9 contributes to lymphatic valve formation and respiratory system maturation. Loss of ITGA9 function in animal models leads to perinatal lethality due to chylothorax, underscoring its essential role in lymphatic and pulmonary development. In adult tissues, Integrin alpha-9 supports wound healing and inflammation by mediating adhesion of leukocytes to endothelial and epithelial cells.

The ITGA9 antibody is used in cell biology, cancer, and immunology research to study integrin-mediated adhesion and signaling. In immunohistochemistry, it detects membrane-associated staining in epithelial and stromal cells. Western blot analysis identifies a ~130 kilodalton band corresponding to the mature glycosylated protein. Integrin alpha-9 participates in epithelial-mesenchymal transition and tumor metastasis, where increased expression correlates with invasive behavior. Blocking ITGA9 function has been shown to suppress cancer cell migration and angiogenesis, highlighting its potential as a therapeutic target.

Integrin alpha-9 interacts with cytoplasmic partners such as talin and kindlin to transmit bidirectional signaling between the ECM and intracellular environment. Activation of this receptor regulates cellular responses including proliferation, differentiation, and immune recruitment. The ITGA9 antibody enables detailed analysis of these signaling events and provides insight into how integrin networks integrate mechanical and chemical cues during tissue remodeling. NSJ Bioreagents provides a validated ITGA9 antibody for its applications, supporting studies in development, inflammation, and cancer metastasis.

UniProt: Q13797