ARHGEF6 antibody (AA 54-765)

Catalog No. ABIN7875657

The Rabbit Polyclonal anti-ARHGEF6 antibody is suitable to detect ARHGEF6 in samples from Human and Mouse. It has been validated for ELISA, WB and FACS.

Quick Overview for ARHGEF6 antibody (AA 54-765) (ABIN7875657)

Target: ARHGEF6 (rho Guanine Nucleotide Exchange Factor (GEF) 6 (ARHGEF6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-ARHGEF6 Antibody

Binding Specificity: AA 54-765

Purpose: ARHGEF6 Antibody / Rho guanine nucleotide exchange factor 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ARHGEF6 recombinant protein (Position: E54-R765) was used as the immunogen for the ARHGEF6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ARHGEF6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ARHGEF6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ARHGEF6

Target: ARHGEF6 (rho Guanine Nucleotide Exchange Factor (GEF) 6 (ARHGEF6))

Alternative Name: ARHGEF6

Background: ARHGEF6 antibody detects Rho guanine nucleotide exchange factor 6, a signaling protein that activates Rho family GTPases to control cytoskeletal organization, cell migration, and synaptic plasticity. The UniProt recommended name is Rho guanine nucleotide exchange factor 6 (ARHGEF6). This protein, also known as alpha-PIX or Cool-2, acts as an exchange factor for RAC1 and CDC42, converting them from GDP-bound inactive forms to GTP-bound active states.

Functionally, ARHGEF6 antibody identifies a 776-amino-acid scaffold protein containing SH3, DH, and PH domains that mediate interaction with PAK kinases and other signaling adaptors. ARHGEF6 promotes actin cytoskeleton rearrangements, lamellipodia formation, and cell adhesion dynamics through activation of Rho GTPase signaling cascades.

The ARHGEF6 gene is located on chromosome Xq26.3 and is highly expressed in the brain, immune cells, and epithelial tissues. It participates in synaptic development, immune cell migration, and epithelial junction organization by coupling surface receptors to cytoskeletal effectors.

Pathologically, mutations in ARHGEF6 cause X-linked intellectual disability due to impaired synaptic signaling and dendritic spine morphology. Aberrant ARHGEF6 expression is also associated with immune dysfunction and metastatic cancer cell migration. Research using ARHGEF6 antibody supports studies in cell signaling, cytoskeletal regulation, and neurological disorders.

ARHGEF6 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect guanine nucleotide exchange factors. NSJ Bioreagents provides ARHGEF6 antibody reagents optimized for studies in signal transduction, neuronal connectivity, and cytoskeletal remodeling.

Structurally, Rho guanine nucleotide exchange factor 6 forms multiprotein complexes with PAK kinases and GIT proteins, linking receptor activation to localized actin assembly. This antibody enables exploration of ARHGEF6's molecular function in Rho GTPase activation and cellular morphogenesis.

UniProt: Q15052

Pathways: Neurotrophin Signaling Pathway