GIGYF1 antibody (AA 55-950)

Catalog No. ABIN7875703

The Rabbit Polyclonal anti-GIGYF1 antibody (ABIN7875703) specifically detects GIGYF1 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for GIGYF1 antibody (AA 55-950) (ABIN7875703)

Target: GIGYF1 (GRB10 Interacting GYF Protein 1 (GIGYF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GIGYF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GIGYF1 Antibody

Binding Specificity: AA 55-950

Purpose: GIGYF1 Antibody / GRB10-interacting GYF protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GIGYF1 recombinant protein (Position: K55-E950) was used as the immunogen for the GIGYF1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GIGYF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GIGYF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GIGYF1

Target: GIGYF1 (GRB10 Interacting GYF Protein 1 (GIGYF1))

Alternative Name: GIGYF1

Background: GIGYF1 antibody detects GRB10-interacting GYF protein 1, encoded by the GIGYF1 gene on chromosome 7q22. GIGYF1 antibody is widely applied in studies of insulin signaling, mRNA regulation, and cell growth. GIGYF1 interacts with GRB10, an adaptor protein in insulin and growth factor receptor pathways, modulating downstream signaling. It also participates in post-transcriptional regulation by binding mRNA cap-binding proteins and translational regulators. Expression is detected in multiple tissues, including brain, pancreas, and muscle, consistent with its roles in metabolism and growth control.

Structurally, GIGYF1 is a protein containing a GYF domain, which recognizes proline-rich motifs, and regions mediating interactions with GRB10 and mRNA-binding proteins. Alternative splicing produces isoforms with variable interaction capacity. Its dual role in signaling and RNA biology reflects structural versatility.

Functionally, GIGYF1 acts as a scaffold linking GRB10 to insulin receptor pathways, regulating PI3K-Akt and MAPK cascades. It also partners with 4EHP, a cap-binding protein, to repress translation of specific mRNAs. Through these mechanisms, GIGYF1 influences metabolism, cell proliferation, and stress responses. Researchers use GIGYF1 antibody to study insulin signaling, translational repression, and metabolic control.

Clinically, GIGYF1 has been implicated in diabetes, neurodevelopmental disorders, and cancer. Genetic variants in GIGYF1 are associated with type 2 diabetes risk and altered glucose homeostasis. Loss-of-function mutations have been linked to developmental delay, autism, and epilepsy, highlighting roles in brain function. Dysregulated expression contributes to oncogenesis, where altered receptor signaling drives proliferation. NSJ Bioreagents supplies GIGYF1 antibody to support studies in endocrinology, neurology, and oncology.

Experimentally, GIGYF1 antibody is used in western blotting to detect the ~160 kDa protein, in immunofluorescence to study subcellular localization, and in immunohistochemistry to evaluate tissue-specific expression. Co-immunoprecipitation with GIGYF1 antibody identifies complexes containing GRB10, 4EHP, and translational regulators.

UniProt: O75420