IdnK antibody (AA 56-182)

Catalog No. ABIN7875761

This Rabbit Polyclonal antibody specifically detects IdnK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for IdnK antibody (AA 56-182) (ABIN7875761)

Target: IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IdnK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IdnK Antibody

Binding Specificity: AA 56-182

Purpose: IDNK Antibody / Gluconokinase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human IDNK recombinant protein (Position: Q56-E182) was used as the immunogen for the IDNK antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IDNK antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IDNK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IdnK

Target: IdnK (IDNK) (IdnK Gluconokinase Homolog (IDNK))

Alternative Name: IDNK

Background: IDNK antibody detects Gluconokinase, an enzyme that catalyzes the phosphorylation of D-gluconate to 6-phosphogluconate in the alternative pentose phosphate pathway. The UniProt recommended name is Gluconokinase (IDNK). This cytosolic enzyme links carbohydrate metabolism with NADPH generation and oxidative stress defense by feeding intermediates into the pentose phosphate shunt.

Functionally, IDNK antibody identifies a 314-amino-acid enzyme that catalyzes the ATP-dependent phosphorylation of gluconate. The reaction product, 6-phosphogluconate, enters the oxidative branch of the pentose phosphate pathway, contributing to NADPH production and ribose biosynthesis. These processes are vital for maintaining redox balance and supporting anabolic metabolism in proliferating cells.

The IDNK gene is located on chromosome 1p36.11 and encodes a kinase belonging to the gluconokinase family. IDNK functions primarily in tissues with active glucose metabolism, such as liver and kidney, where it provides an alternative route for pentose phosphate pathway intermediates. The enzyme's activity is regulated by substrate availability and energy status, integrating carbohydrate metabolism with cellular redox needs.

In metabolic regulation, IDNK contributes to the detoxification of gluconate derived from glucose oxidation and polyol pathway intermediates. It also plays a role in microbial defense and oxidative stress adaptation by maintaining adequate NADPH pools. Altered IDNK expression has been observed in metabolic disorders, cancer, and diabetic tissues, reflecting its role in redox homeostasis and biosynthetic metabolism.

IDNK antibody is widely used in enzymology, metabolism, and redox biology research. It is suitable for immunoblotting, enzyme activity assays, and metabolic flux studies to analyze IDNK expression and function. This antibody supports investigations into pentose phosphate pathway regulation, antioxidant defense, and carbohydrate catabolism. In cancer studies, IDNK detection helps elucidate metabolic rewiring associated with elevated NADPH demand.

Structurally, IDNK adopts an alpha/beta-fold typical of sugar kinases, with conserved ATP-binding and substrate recognition motifs. Its catalytic mechanism involves a sequential binding of ATP and gluconate, followed by phosphoryl transfer. NSJ Bioreagents provides IDNK antibody reagents validated for use in carbohydrate metabolism, redox regulation, and energy balance research.

UniProt: Q5T6J7