Spt6 antibody (AA 564-1639)

Catalog No. ABIN7875791

This Rabbit Polyclonal antibody specifically detects Spt6 in WB, IF, ELISA, ICC and FACS. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Spt6 antibody (AA 564-1639) (ABIN7875791)

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spt6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Spt6 Antibody

Binding Specificity: AA 564-1639

Purpose: SPT6 Antibody / SUPT6H / Transcription elongation factor SPT6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Spt6/SUPT6H recombinant protein (Position: A564-Q1639) was used as the immunogen for the SPT6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPT6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPT6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Alternative Name: SPT6

Background: SPT6 antibody detects Transcription elongation factor SPT6, encoded by the SUPT6H gene located on chromosome 17q11.2. SPT6 is a conserved histone chaperone and transcription elongation factor that couples RNA polymerase II (Pol II) activity to chromatin reassembly. It plays essential roles in transcription elongation, mRNA processing, and maintenance of chromatin integrity during gene expression. SPT6 is expressed in most cell types, with high levels in proliferating tissues and the central nervous system, where active transcription and chromatin remodeling are continuous.

Structurally, SPT6 is a large nuclear protein (~170 kDa) containing tandem SH2 domains at the C-terminus that mediate direct binding to the phosphorylated C-terminal domain of RNA Pol II. The protein also includes acidic and basic regions that interact with nucleosomes, histones H3/H4, and elongation factors. SPT6 belongs to the transcription elongation factor family conserved across eukaryotes and forms complexes with other regulators such as IWS1, PAF1C, and FACT to coordinate chromatin structure with RNA synthesis.

Functionally, SPT6 promotes nucleosome reassembly behind the elongating RNA polymerase and prevents cryptic transcription initiation within coding regions. It also regulates histone modifications, such as H3K36 methylation, by recruiting SETD2 and other histone modifiers during elongation. In addition, SPT6 influences mRNA splicing and 3'-end processing by stabilizing Pol II on gene bodies. Through its dual role as a histone chaperone and elongation factor, SPT6 ensures faithful transcriptional output and epigenetic stability.

Loss or dysregulation of SPT6 disrupts transcription elongation and chromatin organization, leading to genomic instability and aberrant gene expression. Mutations have been associated with developmental defects, cancer, and neurodegenerative disorders. SPT6 also participates in transcription-coupled DNA repair and chromatin restoration after stress. Pathway associations include RNA polymerase II transcription, chromatin remodeling, and epigenetic regulation. During development, SPT6 expression supports neuronal differentiation and transcriptional fidelity in proliferating cells.

The SPT6 antibody from NSJ Bioreagents is an excellent reagent for research into transcription regulation, chromatin maintenance, and epigenetic control.

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response