GLOD4 antibody (AA 57-313)

Catalog No. ABIN7875804

The Rabbit Polyclonal anti-GLOD4 antibody is suitable to detect GLOD4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for GLOD4 antibody (AA 57-313) (ABIN7875804)

Target: GLOD4 (Glyoxalase Domain Containing 4 (GLOD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLOD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GLOD4 Antibody

Binding Specificity: AA 57-313

Purpose: GLOD4 Antibody / Glyoxalase domain-containing protein 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GLOD4 recombinant protein (Position: E57-G313) was used as the immunogen for the GLOD4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GLOD4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GLOD4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GLOD4

Target: GLOD4 (Glyoxalase Domain Containing 4 (GLOD4))

Alternative Name: GLOD4

Background: GLOD4 antibody detects Glyoxalase domain-containing protein 4, encoded by the GLOD4 gene on chromosome 17q25.3. GLOD4 antibody is widely applied in metabolism, detoxification, and mitochondrial biology research. Although GLOD4's precise enzymatic role remains incompletely defined, it contains homology to glyoxalase enzymes, which detoxify reactive dicarbonyls such as methylglyoxal, a byproduct of glycolysis.

Structurally, GLOD4 is a ~36 kDa protein with a glyoxalase-like fold. It is predicted to bind metal ions that assist catalysis, though definitive enzymatic activity has yet to be confirmed. Localization studies suggest enrichment in mitochondria, highlighting a role in redox balance and metabolic regulation.

Functionally, GLOD4 may protect cells from glycation stress by metabolizing reactive aldehydes. Its expression is broad, with higher levels in metabolic tissues such as liver, kidney, and muscle. Researchers use GLOD4 antibody to study mitochondrial metabolism, detoxification pathways, and oxidative stress responses.

Clinically, altered GLOD4 expression has been observed in cancer and metabolic disease. By influencing redox balance, GLOD4 may contribute to tumor survival under stress conditions. It is under investigation as a metabolic biomarker and therapeutic target. NSJ Bioreagents provides GLOD4 antibody for metabolic, mitochondrial, and cancer research.

Experimentally, GLOD4 antibody is applied in western blotting to detect the ~36 kDa protein, in immunofluorescence microscopy to study mitochondrial localization, and in immunohistochemistry to assess expression across tissues. Functional studies combining GLOD4 antibody with metabolic assays help clarify its enzymatic role in glyoxalase pathways.

UniProt: Q9HC38