SMYD1 antibody (AA 57-472)

Catalog No. ABIN7875807

The Rabbit Polyclonal anti-SMYD1 antibody is suitable to detect SMYD1 in samples from Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for SMYD1 antibody (AA 57-472) (ABIN7875807)

Target: SMYD1 (SET and MYND Domain Containing 1 (SMYD1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMYD1 Antibody

Binding Specificity: AA 57-472

Purpose: SMYD1 Antibody / SET and MYND domain-containing protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SMYD1 recombinant protein (Position: K57-Q472) was used as the immunogen for the SMYD1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SMYD1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SMYD1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SMYD1

Target: SMYD1 (SET and MYND Domain Containing 1 (SMYD1))

Alternative Name: SMYD1

Background: SMYD1 antibody detects SET and MYND domain-containing protein 1, a muscle-specific histone methyltransferase that regulates gene expression during cardiac and skeletal muscle differentiation. SMYD1 modulates transcription by methylating histone H3 on lysine 4 and recruiting transcriptional cofactors to promoter regions of muscle-specific genes. The SMYD1 antibody is widely used in developmental biology and epigenetics research to study muscle gene regulation, histone modification, and cardiogenesis.

SMYD1 is encoded by the SMYD1 gene located on human chromosome 2p11.2. The protein is approximately 490 amino acids long and contains an N-terminal SET domain responsible for methyltransferase activity and a C-terminal MYND zinc-finger domain that mediates protein-protein interactions. SMYD1 is predominantly expressed in heart and skeletal muscle tissues, where it coordinates transcriptional programs required for myofibril assembly and contractile function.

The SMYD1 antibody detects a 55 kilodalton band by western blot and shows nuclear and sarcoplasmic staining under immunofluorescence microscopy. SMYD1 acts as a transcriptional regulator of genes encoding structural and contractile proteins such as myosin heavy chains and troponins. It interacts with cofactors like HDACs and skNAC to coordinate repression and activation cycles during myogenesis.

Loss of SMYD1 function in animal models results in defective cardiac morphogenesis, reduced sarcomere organization, and embryonic lethality, highlighting its critical developmental role. Dysregulation of SMYD1 expression has been linked to cardiomyopathy and muscular dystrophy, where abnormal histone methylation alters muscle gene networks. Beyond muscle, SMYD1 may influence differentiation of smooth muscle and endothelial lineages through epigenetic signaling.

Because SMYD1 couples chromatin modification to tissue-specific transcription, it represents a pivotal regulator of muscle differentiation and cardiac development. NSJ Bioreagents provides a validated SMYD1 antibody optimized for its applications, supporting research into epigenetic control, muscle biology, and developmental gene regulation.

UniProt: Q8NB12

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development