DGCR8 antibody (AA 570-771)

Catalog No. ABIN7875818

This Rabbit Polyclonal antibody specifically detects DGCR8 in IF and IHC (p). It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for DGCR8 antibody (AA 570-771) (ABIN7875818)

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DGCR8 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DGCR8 Antibody

Binding Specificity: AA 570-771

Purpose: Zebrafish Dgcr8 Antibody

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Dgcr8 recombinant protein (amino acids K570-Q771) was used as the immunogen for the Zebrafish Dgcr8 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Dgcr8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Dgcr8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DGCR8

Target: DGCR8 (DiGeorge Syndrome Critical Region Gene 8 (DGCR8))

Alternative Name: Dgcr8

Background: Dgcr8, or DiGeorge syndrome critical region gene eight, is a double stranded RNA binding protein that plays a key role in the microRNA processing pathway. In zebrafish, Dgcr8 is an essential component of the microprocessor complex, which includes the ribonuclease enzyme Drosha. Together, they initiate the first step in converting primary microRNA transcripts into precursor microRNAs, which are further processed into mature microRNAs.

MicroRNAs are short noncoding RNAs that regulate gene expression by targeting messenger RNAs for degradation or translational repression. Dgcr8 is crucial for maintaining proper levels of microRNAs, which are involved in numerous biological processes including early development, cell differentiation, neurogenesis, and organ formation.

In zebrafish, Dgcr8 is expressed during early embryogenesis and is required for normal development of the brain, heart, and other organs. Loss of Dgcr8 function results in widespread defects due to disrupted microRNA biogenesis. Because of its evolutionary conservation and central role in RNA regulation, zebrafish Dgcr8 is widely used in studies of gene expression control, developmental biology, and disease models related to microRNA dysfunction.

UniProt: A2BEG3

Pathways: Regulatory RNA Pathways