CYB561 antibody (AA 579-607)

Catalog No. ABIN7875834

The Rabbit Polyclonal anti-CYB561 antibody is suitable to detect CYB561 in samples from Human. It has been validated for WB, FACS and IHC (p).

Quick Overview for CYB561 antibody (AA 579-607) (ABIN7875834)

Target: CYB561 (Cytochrome B-561 (CYB561))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB561 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYB561 Antibody

Binding Specificity: AA 579-607

Purpose: Pyruvate Dehydrogenase E2 Antibody / DLAT

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 579-607 from the human protein was used as the immunogen for the Pyruvate Dehydrogenase E2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Pyruvate Dehydrogenase E2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Pyruvate Dehydrogenase E2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for CYB561

Target: CYB561 (Cytochrome B-561 (CYB561))

Alternative Name: Pyruvate Dehydrogenase E2

Background: DLAT encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95 % of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure.

UniProt: P10515