SPATA6 antibody (AA 58-488)

Catalog No. ABIN7875848

This Rabbit Polyclonal antibody specifically detects SPATA6 in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPATA6 antibody (AA 58-488) (ABIN7875848)

Target: SPATA6 (Spermatogenesis Associated 6 (SPATA6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPATA6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SPATA6 Antibody

Binding Specificity: AA 58-488

Purpose: SPATA6 Antibody / Spermatogenesis-associated protein 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SPATA6 recombinant protein (Position: R58-F488) was used as the immunogen for the SPATA6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPATA6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPATA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPATA6

Target: SPATA6 (Spermatogenesis Associated 6 (SPATA6))

Alternative Name: SPATA6

Background: The SPATA6 antibody targets Spermatogenesis-associated protein 6, a membrane-anchored protein encoded by the SPATA6 gene. Spermatogenesis-associated protein 6 plays a crucial role in sperm tail formation, flagellar assembly, and intercellular junction organization during spermatid maturation. It localizes primarily to the manchette and head-tail coupling apparatus, structures that facilitate shaping and attachment of the developing sperm tail. The SPATA6 antibody allows researchers to study sperm morphogenesis, ciliary structure, and reproductive cell differentiation.

Spermatogenesis-associated protein 6 contributes to the structural integrity of the connecting piece between the nucleus and flagellum, ensuring proper transmission of motility forces. The SPATA6 antibody supports visualization of this protein during spermiogenesis, revealing dynamic redistribution patterns correlated with microtubule organization. Loss of SPATA6 disrupts flagellar biogenesis and causes male infertility in model organisms, emphasizing its essential role in germ-cell architecture.

Beyond testicular expression, Spermatogenesis-associated protein 6 is detected in ciliated epithelial cells, suggesting broader participation in cilium assembly and anchoring. The SPATA6 antibody facilitates these studies and aids in understanding conserved mechanisms that govern axoneme formation and basal-body docking. Its structural role links it to cytoskeletal regulators and motor proteins such as dyneins and kinesins.

Mutations in the SPATA6 gene have been associated with asthenozoospermia, characterized by reduced sperm motility. The SPATA6 antibody enables quantification of protein expression in sperm samples and cell lines, providing valuable diagnostic and mechanistic insights. Additionally, SPATA6 participates in the assembly of intercellular junctions during germ-cell development, influencing Sertolii 1/2spermatid adhesion and polarity.

The SPATA6 antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, typically showing localization to sperm tail and manchette regions. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for reproductive biology and cell-structure research. By enabling accurate detection of Spermatogenesis-associated protein 6, the SPATA6 antibody supports investigations into flagellar morphogenesis, ciliary function, and molecular mechanisms underlying male infertility.

UniProt: Q9NWH7