PREX2 antibody (AA 582-1094)

Catalog No. ABIN7875864

The Rabbit Polyclonal anti-PREX2 antibody is suitable to detect PREX2 in samples from Human and Mouse. It has been validated for ELISA and WB.

Quick Overview for PREX2 antibody (AA 582-1094) (ABIN7875864)

Target: PREX2 (DEPDC2) (DEP Domain Containing 2 (DEPDC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PREX2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-PREX2 Antibody

Binding Specificity: AA 582-1094

Purpose: PREX2 Antibody / DEPDC2

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids D582-Q1094) was used as the immunogen for the PREX2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PREX2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PREX2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PREX2

Target: PREX2 (DEPDC2) (DEP Domain Containing 2 (DEPDC2))

Alternative Name: PREX2

Background: Phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2, also called DEPDC2 (DEP domain-containing protein 2), is a protein that in humans is encoded by the PREX2 gene. The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.

UniProt: Q70Z35