alpha 2 Macroglobulin antibody (AA 604-748)

Catalog No. ABIN7876083

The Mouse Monoclonal anti-alpha 2 Macroglobulin antibody is suitable to detect alpha 2 Macroglobulin in samples from Human. It has been validated for IHC (p).

Quick Overview for alpha 2 Macroglobulin antibody (AA 604-748) (ABIN7876083)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: A2M-6553

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 604-748

Purpose: Alpha-2-Macroglobulin Antibody / A2M

Purification: Protein A/G affinity

Immunogen: A human recombinant A2M protein fragment (within amino acids 604-748) was used as the immunogen for the Alpha-2-Macroglobulin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Alpha-2-Macroglobulin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles

Storage: -20 °C

Storage Comment: Aliquot the Alpha-2-Macroglobulin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: Alpha-2-Macroglobulin

Background: Alpha-2-Macroglobulin antibody targets Alpha-2-Macroglobulin, a large secreted plasma glycoprotein encoded by the A2M gene and a major component of the innate protease inhibition system. Alpha-2-Macroglobulin functions as a broad-spectrum protease inhibitor capable of trapping and inactivating a wide range of proteases, including serine, cysteine, aspartic, and metalloproteases. Through this mechanism, Alpha-2-Macroglobulin plays a critical role in regulating proteolysis, inflammation, and tissue remodeling.

Alpha-2-Macroglobulin is synthesized primarily by hepatocytes and secreted into the bloodstream, where it circulates at high concentrations. It is also produced locally by macrophages, fibroblasts, and certain epithelial cells under inflammatory or stress conditions. As a soluble extracellular protein, Alpha-2-Macroglobulin interacts with proteases via a bait region that triggers a conformational change, physically entrapping the protease and targeting the complex for clearance through receptor-mediated endocytosis. A2M antibody detection is therefore relevant for studies of extracellular protease regulation and immune surveillance.

Beyond protease inhibition, Alpha-2-Macroglobulin functions as a carrier protein for cytokines, growth factors, and hormones, including transforming growth factor beta and basic fibroblast growth factor. By binding and modulating the bioavailability of these signaling molecules, Alpha-2-Macroglobulin influences processes such as cell proliferation, wound healing, and immune responses. A2M antibody reagents support investigations into how protease control intersects with growth factor signaling and inflammatory regulation.

Dysregulation of Alpha-2-Macroglobulin expression or function has been associated with numerous disease states. Altered A2M levels have been reported in liver disease, neurodegenerative disorders, cardiovascular disease, and cancer. In the nervous system, Alpha-2-Macroglobulin has been implicated in amyloid beta clearance and neuroinflammation, while in oncology it may influence tumor progression by modulating protease activity within the tumor microenvironment. These diverse biological roles underscore the value of A2M antibody-based detection in both basic and translational research.

Clone A2M/6553 is designed to recognize Alpha-2-Macroglobulin in research applications. Alpha-2-Macroglobulin antibody reagents are suitable for detecting protein expression and distribution in serum, tissues, and cell-derived samples, supporting studies focused on protease regulation, inflammatory processes, and disease-associated alterations in extracellular protein homeostasis.

UniProt: P01023

Pathways: Lipid Metabolism