Melanophilin antibody (AA 61-523)

Catalog No. ABIN7876110

The Rabbit Polyclonal anti-Melanophilin antibody is suitable to detect Melanophilin in samples from Human. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for Melanophilin antibody (AA 61-523) (ABIN7876110)

Target: Melanophilin (MLPH)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Melanophilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Melanophilin Antibody

Binding Specificity: AA 61-523

Purpose: MLPH Antibody / Melanophilin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Melanophilin/MLPH recombinant protein (Position: E61-L523) was used as the immunogen for the MLPH antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MLPH antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MLPH antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Melanophilin

Target: Melanophilin (MLPH)

Alternative Name: MLPH

Background: MLPH antibody detects Melanophilin, a Rab effector protein that regulates melanosome transport and distribution within pigment cells. Encoded by the MLPH gene on chromosome 2q37.3, this protein functions as an adaptor linking Rab27A and myosin Va to form a tripartite complex essential for actin-based organelle transport. Melanophilin ensures that pigment-containing melanosomes are properly positioned within melanocytes for uniform pigment dispersion and transfer to keratinocytes.

Structurally, Melanophilin is a 590-amino-acid cytoplasmic protein of approximately 67 kilodaltons containing an N-terminal Rab27A-binding domain, a central actin- and myosin Va-interacting region, and a C-terminal coiled-coil domain that stabilizes the tripartite complex. This structural organization enables MLPH to bridge the small GTPase Rab27A and the motor protein myosin Va, coordinating microtubule and actin-based transport systems. In pigment cells, MLPH localizes to melanosome membranes and cortical actin filaments where it regulates vesicle tethering and release.

The MLPH antibody is widely used in cell biology, dermatology, and neurobiology research to study vesicle trafficking, motor protein coordination, and pigment cell organization. Western blot analysis detects a 67 kilodalton band corresponding to Melanophilin, while immunofluorescence reveals peripheral punctate staining in melanocytes, consistent with melanosomal distribution. This antibody is ideal for examining organelle transport mechanisms and intracellular vesicle dynamics in epithelial and neuronal systems.

Mutations in MLPH cause Griscelli syndrome type 3, a rare pigmentation disorder characterized by hypopigmentation and abnormal melanosome aggregation due to disrupted melanosome transport. Functional studies show that loss of MLPH leads to perinuclear melanosome clustering, highlighting its importance in vesicle tethering to the actin cytoskeleton. Beyond pigmentation, MLPH has been implicated in secretory vesicle dynamics in non-pigment cell types, suggesting a broader role in vesicle trafficking and cytoskeletal coupling. The MLPH antibody provides a reliable tool for characterizing this transport system and investigating the molecular basis of vesicle transport defects. NSJ Bioreagents validates this antibody for its applications, ensuring high specificity and reproducibility for vesicle trafficking and cytoskeletal research.

UniProt: Q9BV36