PHKG1 antibody (AA 62-353)

Catalog No. ABIN7876159

The Rabbit Polyclonal anti-PHKG1 antibody (ABIN7876159) specifically detects PHKG1 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for PHKG1 antibody (AA 62-353) (ABIN7876159)

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHKG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PHKG1 Antibody

Binding Specificity: AA 62-353

Purpose: PHKG1 Antibody / Phosphorylase kinase gamma 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PHKG1 recombinant protein (Position: E62-R353) was used as the immunogen for the PHKG1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PHKG1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PHKG1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PHKG1

Target: PHKG1 (phosphorylase Kinase, gamma 1 (Muscle) (PHKG1))

Alternative Name: PHKG1

Background: PHKG1 antibody detects Phosphorylase kinase gamma 1, a catalytic subunit of the phosphorylase kinase (PhK) complex that plays a central role in glycogen metabolism. The UniProt recommended name is Phosphorylase kinase gamma 1 (PHKG1). This enzyme functions as a serine/threonine-specific protein kinase responsible for activating glycogen phosphorylase, the enzyme that catalyzes the rate-limiting step in glycogen breakdown. PHKG1 serves as the catalytic component within the multimeric PhK complex, which also includes alpha, beta, and delta subunits that provide regulatory and calcium-binding functions.

Phosphorylase kinase gamma 1 is predominantly expressed in skeletal muscle, where it regulates glycogenolysis in response to hormonal and neural stimulation. Activation of the enzyme occurs through phosphorylation and calcium binding, linking it directly to adrenergic and calcium signaling pathways. The PhK complex functions as a molecular bridge between intracellular calcium release and the mobilization of energy reserves during muscle contraction, ensuring that glucose-1-phosphate is made available for rapid ATP production.

Structurally, PHKG1 contains conserved kinase domains typical of the protein kinase superfamily, including the ATP-binding site and catalytic loop that mediates phosphate transfer. The gamma 1 isoform differs from the gamma 2 isoform (encoded by PHKG2), which is more abundant in liver tissue, highlighting its tissue-specific regulation. The PHKG1 gene is located on chromosome 7p11.2 and its expression is tightly regulated according to metabolic demand and muscle fiber type.

Defects or mutations in PHKG1 can lead to glycogen storage disease type IXd, a rare inherited metabolic disorder characterized by reduced phosphorylase kinase activity, muscle weakness, and exercise intolerance. Because of its key role in energy metabolism, PHKG1 is also investigated in studies of muscle physiology, insulin signaling, and exercise adaptation. Detection of Phosphorylase kinase gamma 1 using PHKG1 antibody allows researchers to monitor enzyme expression, activity, and localization in muscle and metabolic tissues.

PHKG1 antibody is useful for applications such as immunohistochemistry, immunofluorescence, and related assays examining kinase regulation and energy metabolism. By identifying the catalytic subunit of phosphorylase kinase, this antibody supports research into glycogen regulation, muscle energy dynamics, and kinase-mediated signaling networks. NSJ Bioreagents provides PHKG1 antibody reagents optimized for studies of glycogen metabolism and skeletal muscle biochemistry.

UniProt: Q16816

Pathways: Cellular Glucan Metabolic Process