OTUD6B antibody (AA 66-293)

Catalog No. ABIN7876335

The Rabbit Polyclonal anti-OTUD6B antibody is suitable to detect OTUD6B in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for OTUD6B antibody (AA 66-293) (ABIN7876335)

Target: OTUD6B (OTU Domain Containing 6B (OTUD6B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OTUD6B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-OTUD6B Antibody

Binding Specificity: AA 66-293

Purpose: OTUD6B Antibody / OTU domain-containing deubiquitinase 6B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human OTUD6B recombinant protein (Position: E66-S293) was used as the immunogen for the OTUD6B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OTUD6B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OTUD6B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for OTUD6B

Target: OTUD6B (OTU Domain Containing 6B (OTUD6B))

Alternative Name: OTUD6B

Background: OTUD6B antibody detects OTU domain-containing deubiquitinase 6B, a cysteine protease encoded by the OTUD6B gene located on chromosome 8q21.3. OTUD6B belongs to the ovarian tumor (OTU) domain-containing deubiquitinase family, which removes ubiquitin moieties from target proteins to regulate their stability, localization, and signaling activity. OTUD6B plays an important role in cellular protein homeostasis, DNA damage repair, and immune signaling by fine-tuning ubiquitin-mediated pathways. It is expressed in most tissues, with highest levels in brain, liver, and testis, reflecting its broad regulatory functions.

Structurally, OTUD6B contains an N-terminal OTU catalytic domain characterized by a conserved cysteine, histidine, and aspartate catalytic triad responsible for cleaving ubiquitin from substrates. It also includes regions for substrate recognition and potential regulatory phosphorylation sites. OTUD6B belongs to the OTU-type deubiquitinase family, which exhibits linkage-specific cleavage of polyubiquitin chains. Co-localization studies show OTUD6B primarily in the cytoplasm but also associated with the nucleus under stress conditions, suggesting roles in both cytosolic and nuclear signaling pathways.

Functionally, OTUD6B regulates protein degradation and signaling by removing ubiquitin from specific substrates targeted for proteasomal or lysosomal turnover. It modulates the stability of signaling mediators such as AKT, p53, and components of the mTOR pathway. OTUD6B also contributes to endoplasmic reticulum stress responses and DNA damage repair by reversing ubiquitination on repair factors. In immune cells, OTUD6B helps maintain homeostasis by controlling NF-?B activation and cytokine signaling. Known biochemical activities include cleavage of Lys48- and Lys63-linked ubiquitin chains, thereby influencing protein degradation and signaling transduction.

Deficiency or mutation of OTUD6B has been associated with intellectual developmental disorders, craniofacial abnormalities, and growth retardation, collectively known as OTUD6B-related neurodevelopmental syndrome. Dysregulation of OTUD6B expression has also been linked to tumor progression through effects on apoptosis and metabolic control. Pathway associations include ubiquitin-dependent protein catabolism, mTOR signaling, and cellular stress response. During development, OTUD6B supports cell growth and neuronal maturation by regulating key ubiquitinated targets involved in metabolism and transcription.

The OTUD6B antibody from NSJ Bioreagents is an excellent reagent for research on deubiquitination mechanisms, proteostasis, and signal transduction regulation.

UniProt: Q8N6M0