Chordin antibody (AA 69-940)

Catalog No. ABIN7876452

The Rabbit Polyclonal anti-Chordin antibody is suitable to detect Chordin in samples from Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for Chordin antibody (AA 69-940) (ABIN7876452)

Target: Chordin (CHRD)

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chordin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Chordin Antibody

Binding Specificity: AA 69-940

Purpose: Zebrafish Chordin Antibody / Chd / Chrd

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Chordin recombinant protein (amino acids H69-H940) was used as the immunogen for the Zebrafish Chordin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Chordin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Chordin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Chordin

Target: Chordin (CHRD)

Alternative Name: Chordin

Background: Chordin (also called Chd, Chrd) is a secreted glycoprotein that plays a central role in early embryonic development by antagonizing Bone Morphogenetic Protein (BMP) signaling. In zebrafish (Danio rerio), Chordin is a key dorsalizing factor that helps establish the dorsoventral axis during gastrulation, contributing to proper tissue patterning and organogenesis.

Chordin functions by binding directly to BMP ligands (such as Bmp2 and Bmp4), preventing them from interacting with their receptors and thereby inhibiting BMP signaling activity in dorsal regions of the embryo. The zebrafish chordin gene is expressed in the organizer region (shield) during early development, and its activity is essential for the formation of dorsal structures such as the notochord, neural plate, and somites.

Loss-of-function mutations in chordin lead to ventralized phenotypes, while overexpression can result in dorsalization, underscoring its importance as a morphogen regulator. Due to its highly conserved function across vertebrates, zebrafish Chordin is widely used in studies of axis specification, morphogen gradients, neural induction, and BMP pathway modulation.

UniProt: O57472