Nestin antibody (AA 698-844)

Catalog No. ABIN7876460

This Mouse Monoclonal antibody specifically detects Nestin in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Nestin antibody (AA 698-844) (ABIN7876460)

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NES-2911

Product Details anti-Nestin Antibody

Binding Specificity: AA 698-844

Purpose: Nestin Antibody

Purification: Protein G affinity chromatography

Immunogen: A recombinant human partial protein (amino acids 698-844) was used as the immunogen for the Nestin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Nestin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Nestin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.

UniProt: P48681