RHBG antibody (AA 7-394)

Catalog No. ABIN7876495

The Rabbit Polyclonal anti-RHBG antibody (ABIN7876495) specifically detects RHBG in WB, FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for RHBG antibody (AA 7-394) (ABIN7876495)

Target: RHBG

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHBG antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-RHBG Antibody

Binding Specificity: AA 7-394

Purpose: RHBG Antibody / Ammonium transporter Rh type B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RHBG recombinant protein (Position: R7-L394) was used as the immunogen for the RHBG antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RHBG antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RHBG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RHBG

Target: RHBG

Background: RHBG antibody detects Ammonium transporter Rh type B, a member of the Rhesus glycoprotein family that mediates ammonia and ammonium transport across cell membranes. The UniProt recommended name is Ammonium transporter Rh type B (RHBG). This protein functions as a bidirectional transporter of ammonia (NH3) and plays a crucial role in maintaining acid-base balance and nitrogen metabolism, particularly in the kidney and liver.

Functionally, RHBG antibody identifies a 458-amino-acid multi-pass transmembrane protein localized to the basolateral membrane of epithelial cells in renal collecting ducts and hepatocytes. RHBG facilitates the diffusion of ammonia across cell membranes, coupling nitrogen excretion with pH homeostasis. It functions as part of the ammonia recycling pathway that adjusts renal acid secretion according to metabolic conditions. RHBG complements the activity of the related transporter RHCG, which operates on the apical membrane of renal tubular cells to mediate ammonia excretion into urine.

The RHBG gene is located on chromosome 1q21.3 and encodes a protein containing 12 predicted transmembrane domains typical of ammonium transporters. Structural studies indicate that RHBG operates as a gas channel rather than an ion exchanger, selectively permeable to ammonia. Its expression is regulated by systemic acid-base status, hormonal control, and dietary nitrogen intake. In the liver, RHBG contributes to ammonia detoxification by facilitating its diffusion into hepatocytes for conversion to urea via the urea cycle.

Disruption of RHBG function leads to impaired ammonia transport and metabolic acidosis. Altered expression has been observed in chronic kidney disease and hepatic encephalopathy, conditions characterized by disordered nitrogen metabolism. In addition to its renal and hepatic roles, RHBG is expressed in brain, skin, and reproductive tissues, where it contributes to local pH and nitrogen regulation. Its regulation by hypoxia-inducible factors (HIFs) and glucocorticoids underscores its involvement in physiological adaptation to metabolic stress.

RHBG antibody is widely used in renal physiology, hepatology, and membrane transport research. It is suitable for immunoblotting, immunohistochemistry, and confocal microscopy to analyze RHBG expression and localization. In kidney research, this antibody helps distinguish basolateral ammonia transport from apical excretion mechanisms. Its detection supports studies on acid-base homeostasis, ammonia metabolism, and renal tubular function. RHBG also serves as a biomarker for nitrogen-handling capacity in metabolic and toxicological studies.

Structurally, RHBG belongs to the Amt/Mep/Rh superfamily of ammonia transporters. It forms homotrimers in the membrane, each subunit containing a hydrophobic pore that selectively channels ammonia. Post-translational modifications such as glycosylation influence its membrane stability and transport efficiency. NSJ Bioreagents provides RHBG antibody reagents validated for research in ammonia transport, renal physiology, and metabolic regulation.

UniProt: Q9H310