STXBP3 antibody (AA 7-592)

Catalog No. ABIN7876509

The Rabbit Polyclonal anti-STXBP3 antibody is suitable to detect STXBP3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and FACS.

Quick Overview for STXBP3 antibody (AA 7-592) (ABIN7876509)

Target: STXBP3 (Syntaxin Binding Protein 3 (STXBP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STXBP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-STXBP3 Antibody

Binding Specificity: AA 7-592

Purpose: STXBP3 Antibody / Syntaxin binding protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human STXBP3 recombinant protein (Position: E7-E592) was used as the immunogen for the STXBP3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the STXBP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the STXBP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for STXBP3

Target: STXBP3 (Syntaxin Binding Protein 3 (STXBP3))

Alternative Name: STXBP3

Background: STXBP3 antibody recognizes Syntaxin binding protein 3, a member of the Sec1/Munc18-like (SM) family of proteins that regulate vesicle fusion and exocytosis. The STXBP3 gene encodes a cytosolic protein also known as Munc18c, which interacts with syntaxins, particularly Syntaxin 4, to mediate docking and fusion of vesicles with the plasma membrane. This interaction is critical for regulated exocytosis in diverse cell types including adipocytes, pancreatic beta cells, neurons, and epithelial cells. By controlling SNARE complex assembly, STXBP3 ensures proper timing of vesicle release during insulin secretion, neurotransmission, and cell-surface trafficking of key receptors and transporters.

STXBP3 plays a central role in glucose homeostasis. In adipose and muscle tissue, Munc18c regulates insulin-stimulated translocation of glucose transporter 4 (GLUT4) to the plasma membrane, an essential step in glucose uptake. Dysregulation or reduced expression of STXBP3 has been associated with insulin resistance and type 2 diabetes. In pancreatic beta cells, STXBP3 interacts with Syntaxin 1A and VAMP2 to influence insulin granule exocytosis. Beyond metabolic regulation, STXBP3 participates in vesicle trafficking in epithelial tissues and contributes to cell polarity maintenance and junctional organization.

The human STXBP3 gene is located on chromosome 1p36.23 and contains multiple alternatively spliced isoforms. Protein structure analysis reveals that STXBP3 comprises three domains that form a closed conformation around its syntaxin partners, preventing premature SNARE complex formation. This inhibitory mechanism ensures vesicles only fuse in response to proper signaling cues. The protein's expression is ubiquitous but enriched in secretory tissues and endocrine glands. STXBP3 has also been implicated in immune responses through regulation of vesicle-mediated cytokine release.

Immunohistochemical staining with STXBP3 antibody demonstrates cytoplasmic localization in skeletal muscle, pancreatic islets, and epithelial cells. It serves as a valuable reagent for studies of insulin signaling, exocytosis, vesicular transport, and metabolic regulation. The STXBP3 antibody from NSJ Bioreagents provides a versatile tool for examining vesicle fusion mechanisms and SNARE complex dynamics across various cellular systems.

UniProt: O00186

Pathways: Hormone Transport