GAD antibody (AA 72-135)

Catalog No. ABIN7876622

This Mouse Monoclonal antibody specifically detects GAD in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for GAD antibody (AA 72-135) (ABIN7876622)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: GAD1-2391

Product Details anti-GAD Antibody

Binding Specificity: AA 72-135

Purpose: GAD67 Antibody / GAD1 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the GAD67 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the GAD67 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD67

Background: This mAb recognizes a protein of 67 kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

UniProt: Q99259