RBM42 antibody (AA 73-469)

Catalog No. ABIN7876681

The Rabbit Polyclonal anti-RBM42 antibody (ABIN7876681) specifically detects RBM42 in WB, ELISA, IHC, FACS, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RBM42 antibody (AA 73-469) (ABIN7876681)

Target: RBM42 (RNA Binding Motif Protein 42 (RBM42))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM42 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RBM42 Antibody

Binding Specificity: AA 73-469

Purpose: RBM42 Antibody / RNA-binding motif protein 42

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RBM42 recombinant protein (Position: E73-K469) was used as the immunogen for the RBM42 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RBM42 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RBM42 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RBM42

Target: RBM42 (RNA Binding Motif Protein 42 (RBM42))

Alternative Name: RBM42

Background: RBM42 antibody detects RNA-binding motif protein 42, a nuclear RNA-binding protein involved in post-transcriptional gene regulation, mRNA stability, and stress response. Encoded by the RBM42 gene on chromosome 12q13.12, this protein belongs to the RNA recognition motif (RRM) family and participates in alternative splicing, RNA transport, and translation control. RBM42 plays an important role in maintaining transcriptome stability during stress and in modulating the fate of specific mRNAs involved in cell growth and survival.

Structurally, RBM42 contains two conserved RRM domains that enable specific binding to U-rich RNA sequences. It interacts with the spliceosome and ribonucleoprotein complexes to regulate pre-mRNA processing. During cellular stress, RBM42 localizes to stress granules and contributes to the sequestration of mRNAs, preventing unwanted translation and preserving RNA integrity until conditions normalize. It also associates with components of the nonsense-mediated decay pathway, suggesting a role in RNA surveillance and turnover.

The RBM42 antibody is widely used in molecular biology, RNA processing, and stress signaling research to study post-transcriptional control mechanisms and RNA-protein complex dynamics. Western blot analysis identifies a 45 kilodalton band corresponding to RBM42, while immunofluorescence reveals strong nuclear staining with perinucleolar enrichment under basal conditions. Upon stress induction, cytoplasmic granule localization becomes prominent, consistent with its role in RNA regulation and cellular adaptation.

RBM42 is implicated in diverse biological processes, including embryonic development, hematopoiesis, and tumor progression. Its expression is upregulated under hypoxia and oxidative stress, contributing to cellular resistance mechanisms. Dysregulation of RBM42 has been associated with aberrant mRNA metabolism in cancers and neurodegenerative diseases, where mis-splicing and altered RNA transport disrupt homeostasis. The RBM42 antibody enables researchers to track these dynamic changes and characterize its role in RNA fate determination.

Functionally, RBM42 acts as a molecular chaperone for specific mRNAs, maintaining their stability and coordinating translation with cellular energy states. It interfaces with the ribosomal machinery and modulates translation initiation through mRNA secondary structure binding. The RBM42 antibody provides a powerful reagent for exploring RNA biology, splicing regulation, and cellular stress adaptation. NSJ Bioreagents validates this antibody for western blotting, immunohistochemistry, and immunofluorescence, ensuring high performance and reproducibility in RNA-related research.

UniProt: Q9BTD8