Syntaxin 10 antibody (AA 77-218)

Catalog No. ABIN7876759

The Rabbit Polyclonal anti-Syntaxin 10 antibody (ABIN7876759) specifically detects Syntaxin 10 in ELISA, WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Syntaxin 10 antibody (AA 77-218) (ABIN7876759)

Target: Syntaxin 10 (STX10)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syntaxin 10 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Syntaxin 10 Antibody

Binding Specificity: AA 77-218

Purpose: STX10 Antibody / Syntaxin 10

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Syntaxin 10/STX10 recombinant protein (Position: K77-K218) was used as the immunogen for the STX10 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the STX10 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the STX10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Syntaxin 10

Target: Syntaxin 10 (STX10)

Alternative Name: STX10

Background: STX10 antibody detects Syntaxin-10, a member of the t-SNARE family involved in vesicle trafficking and membrane fusion within the endosomal system. Encoded by the STX10 gene on chromosome 19q13.32, this protein localizes primarily to the trans-Golgi network (TGN) and endosomes, where it regulates cargo recycling and sorting. Syntaxin-10 interacts with other SNARE proteins, including Vti1a, VAMP3, and STX16, forming complexes that mediate vesicle fusion events critical for protein trafficking between the Golgi apparatus and endosomal compartments.

STX10 is essential for maintaining endosomal organization and cargo specificity, particularly in the recycling of mannose 6-phosphate receptors (MPRs) that deliver lysosomal hydrolases. It contributes to the tethering and fusion of vesicles returning from late endosomes to the TGN, a process required for proper lysosome formation and enzyme sorting. Loss of STX10 function disrupts endosomal integrity and leads to defective lysosomal enzyme trafficking, underscoring its role in vesicle-mediated transport.

The STX10 antibody is widely used in cell biology, membrane trafficking, and neurobiology research to investigate SNARE-mediated vesicular transport. Western blot analysis identifies a 30 kilodalton band corresponding to Syntaxin-10, while immunofluorescence reveals punctate perinuclear staining that colocalizes with Golgi and endosomal markers such as TGN46 and Rab9. This antibody supports detailed analysis of intracellular trafficking, organelle organization, and vesicle fusion mechanisms.

Beyond its role in vesicle trafficking, STX10 participates in endosome-to-Golgi transport pathways that maintain protein homeostasis and receptor recycling. It also contributes to specialized trafficking functions in neurons, immune cells, and epithelial tissues. Dysregulation of SNARE complex components, including STX10, has been implicated in neurodegenerative diseases and metabolic disorders where vesicle trafficking pathways are compromised. The STX10 antibody enables researchers to explore these processes and assess Syntaxin-10 function in both normal and pathological conditions. NSJ Bioreagents provides this antibody validated for its applications, ensuring accurate and reproducible detection for studies of intracellular trafficking and membrane fusion.

UniProt: O60499