TRIM2 antibody (AA 78-398)

Catalog No. ABIN7876784

This Rabbit Polyclonal antibody specifically detects TRIM2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRIM2 antibody (AA 78-398) (ABIN7876784)

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TRIM2 Antibody

Binding Specificity: AA 78-398

Purpose: TRIM2 Antibody / Tripartite motif-containing protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TRIM2 recombinant protein (Position: Q78-E398) was used as the immunogen for the TRIM2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TRIM2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TRIM2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TRIM2

Target: TRIM2 (Tripartite Motif Containing 2 (TRIM2))

Alternative Name: TRIM2

Background: TRIM2 antibody detects Tripartite motif-containing protein 2, an E3 ubiquitin ligase that regulates neuronal cytoskeleton organization and axonal stability. TRIM2 belongs to the TRIM family of RING finger E3 ligases and mediates ubiquitination of neurofilament proteins and signaling regulators. The TRIM2 antibody is widely used in neuroscience, developmental, and cell biology research to study axon maintenance, neurodegeneration, and ubiquitin-mediated proteostasis.

TRIM2 is encoded by the TRIM2 gene located on human chromosome 4q31.3. The protein is approximately 744 amino acids long and contains a RING finger domain, two B-boxes, a coiled-coil region, and a C-terminal filamin-binding NHL repeat domain. TRIM2 localizes to axons, growth cones, and perinuclear regions, reflecting its role in neuronal trafficking and cytoskeletal regulation.

The TRIM2 antibody detects a 90 kilodalton band by western blot and reveals filamentous cytoplasmic staining under immunofluorescence microscopy. TRIM2 ubiquitinates neurofilament light chain (NEFL) to control filament organization and turnover, maintaining axonal integrity and preventing neurodegenerative aggregation. It also regulates apoptosis by targeting proapoptotic proteins for degradation and interacts with signaling pathways involved in neuronal survival.

Mutations in TRIM2 cause hereditary axonal neuropathy and neurodegenerative disease characterized by axon swelling and degeneration. Dysregulation of TRIM2 expression has also been linked to gliomas and other cancers, where altered ubiquitination affects cytoskeletal dynamics and invasion. In developing neurons, TRIM2 coordinates cytoskeletal remodeling during axon guidance and branching.

Because of its key role in ubiquitin-mediated regulation of the neuronal cytoskeleton, TRIM2 serves as an essential molecule for understanding axonal maintenance and neuroprotection. NSJ Bioreagents provides a validated TRIM2 antibody optimized for its applications, supporting studies of ubiquitination, neurodevelopment, and axon integrity.

UniProt: Q9C040