PDZK1 antibody (AA 8-502)

Catalog No. ABIN7876837

The Rabbit Polyclonal anti-PDZK1 antibody (ABIN7876837) specifically detects PDZK1 in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDZK1 antibody (AA 8-502) (ABIN7876837)

Target: PDZK1 (PDZ Domain Containing 1 (PDZK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDZK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-PDZK1 Antibody

Binding Specificity: AA 8-502

Purpose: PDZK1 Antibody / PDZ domain-containing 1 / NHERF3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse PDZK1 recombinant protein (Position: R8-R502) was used as the immunogen for the PDZK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDZK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PDZK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDZK1

Target: PDZK1 (PDZ Domain Containing 1 (PDZK1))

Alternative Name: PDZK1

Background: PDZK1 antibody detects PDZ domain-containing 1, a scaffolding protein that organizes membrane transporters and receptors in epithelial cells. The UniProt recommended name is PDZ domain-containing 1 (PDZK1). This adaptor protein interacts with various membrane-associated proteins, including ion channels, transporters, and signaling receptors, to regulate their surface expression and stability.

Functionally, PDZK1 antibody identifies a 519-amino-acid cytoplasmic protein containing four PDZ domains that mediate multiprotein complex formation at the apical membrane. PDZK1 anchors transporters such as the sodium-hydrogen exchanger NHE3 and the scavenger receptor SR-BI, facilitating ion exchange and lipid uptake. It coordinates interactions between membrane proteins and signaling molecules that maintain epithelial polarity and transport efficiency.

The PDZK1 gene is located on chromosome 1q21.1 and is highly expressed in kidney, liver, and intestine. In hepatocytes, PDZK1 regulates cholesterol homeostasis through its interaction with SR-BI, while in renal and intestinal epithelia it modulates absorption and reabsorption processes. PDZK1's scaffolding role ensures efficient communication between transporters and cytoskeletal components.

Pathologically, dysregulated PDZK1 expression is linked to disorders of lipid metabolism, hypertension, and cancer. Reduced PDZK1 can impair high-density lipoprotein (HDL) uptake and disrupt cholesterol transport. Overexpression has been associated with tumor progression through altered signaling dynamics. Research using PDZK1 antibody enhances understanding of epithelial transport regulation and metabolic signaling.

PDZK1 antibody is suitable for western blotting, immunofluorescence, and immunohistochemistry to detect PDZK1 in epithelial tissues and cultured cells. NSJ Bioreagents supplies validated PDZK1 antibody reagents for studies of membrane trafficking, ion transport, and lipid metabolism.

Structurally, PDZK1 features four PDZ domains arranged to allow simultaneous binding of multiple partners, forming scaffolded microdomains at the plasma membrane. This antibody enables analysis of PDZK1-mediated protein complexes that maintain cellular polarity and metabolic balance.

UniProt: Q9JIL4