WISP1 antibody (AA 80-367)

Catalog No. ABIN7876860

The Rabbit Polyclonal anti-WISP1 antibody is suitable to detect WISP1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for WISP1 antibody (AA 80-367) (ABIN7876860)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WISP1 Antibody

Binding Specificity: AA 80-367

Purpose: CCN4 Antibody / WISP1

Purification: Antigen affinity purified

Immunogen: Recombinant human WISP-1/CCN4 protein (amino acids A80-N367) was used as the immunogen for the CCN4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CCN4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CCN4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: CCN4

Background: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.

UniProt: O95388

Pathways: WNT Signaling, Growth Factor Binding