POLDIP2 antibody (AA 81-324)

Catalog No. ABIN7876894

The Rabbit Polyclonal anti-POLDIP2 antibody (ABIN7876894) specifically detects POLDIP2 in WB, ELISA, IP, IF, FACS and ICC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for POLDIP2 antibody (AA 81-324) (ABIN7876894)

Target: POLDIP2 (Polymerase (DNA-Directed), delta Interacting Protein 2 (POLDIP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLDIP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-POLDIP2 Antibody

Binding Specificity: AA 81-324

Purpose: POLDIP2 Antibody / Polymerase delta-interacting protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human POLDIP2 recombinant protein (Position: Q81-Q324) was used as the immunogen for the POLDIP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the POLDIP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the POLDIP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for POLDIP2

Target: POLDIP2 (Polymerase (DNA-Directed), delta Interacting Protein 2 (POLDIP2))

Alternative Name: POLDIP2

Background: POLDIP2 antibody detects Polymerase delta-interacting protein 2, a multifunctional protein that regulates DNA replication, repair, and mitochondrial function. The UniProt recommended name is Polymerase delta-interacting protein 2 (POLDIP2). This adaptor protein enhances the activity of DNA polymerase delta and coordinates cellular redox balance, cytoskeletal organization, and oxidative stress responses.

Functionally, POLDIP2 antibody identifies a 368-amino-acid protein localized to the nucleus, cytoplasm, and mitochondria. POLDIP2 binds to DNA polymerase delta subunit p50, stimulating processive DNA synthesis. It also interacts with NADPH oxidase 4 (NOX4), promoting the production of reactive oxygen species for signaling and host defense. In mitochondria, POLDIP2 influences respiratory complex stability and oxidative phosphorylation efficiency.

The POLDIP2 gene is located on chromosome 17q11.2 and is broadly expressed in metabolically active tissues, including heart, liver, and brain. Its expression is induced by stress signals and growth factors, integrating DNA repair, redox signaling, and metabolic regulation.

Pathologically, altered POLDIP2 expression has been linked to vascular remodeling, fibrosis, cancer, and neurodegenerative disorders. Overexpression promotes proliferation and migration in vascular smooth muscle cells, while deficiency impairs mitochondrial respiration and DNA replication fidelity. Research using POLDIP2 antibody supports studies in DNA metabolism, oxidative stress, and mitochondrial biology.

POLDIP2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect replication-associated and redox-regulating proteins. NSJ Bioreagents provides POLDIP2 antibody reagents optimized for research in DNA repair, metabolism, and redox signaling.

Structurally, Polymerase delta-interacting protein 2 contains an N-terminal mitochondrial targeting sequence and a YccV-like domain involved in protein-protein interactions. This antibody facilitates investigation of POLDIP2's role in coordinating nuclear and mitochondrial stress responses.

UniProt: Q9Y2S7