LPAR5 antibody (AA 83-372)

Catalog No. ABIN7876940

This Rabbit Polyclonal antibody specifically detects LPAR5 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for LPAR5 antibody (AA 83-372) (ABIN7876940)

Target: LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPAR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-LPAR5 Antibody

Binding Specificity: AA 83-372

Purpose: LPAR5 Antibody / Lysophosphatidic acid receptor 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human LPAR5 recombinant protein (Position: A83-L372) was used as the immunogen for the LPAR5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the LPAR5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the LPAR5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for LPAR5

Target: LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))

Alternative Name: LPAR5

Background: LPAR5 antibody detects Lysophosphatidic acid receptor 5, a G protein-coupled receptor (GPCR) that mediates signaling by lysophosphatidic acid (LPA), a bioactive phospholipid involved in cell proliferation, motility, and immune regulation. The UniProt recommended name is Lysophosphatidic acid receptor 5 (LPAR5). This receptor couples primarily to G12/13 and Gq proteins, triggering downstream activation of Rho and phospholipase C pathways.

Functionally, LPAR5 antibody identifies a 353-amino-acid receptor localized to the plasma membrane. LPAR5 activation induces calcium mobilization, cytoskeletal rearrangement, and cytokine release. It contributes to pain sensation, vascular permeability, and immune cell migration. In neuronal cells, LPAR5 influences axonal guidance and neuroinflammation.

The LPAR5 gene is located on chromosome 12p13.31 and is expressed in platelets, lymphocytes, dorsal root ganglia, and intestinal epithelial cells. It plays roles in both physiological and pathological processes such as wound healing, sensory transmission, and inflammation.

Pathologically, dysregulation of LPAR5 signaling has been implicated in neuropathic pain, cancer metastasis, and autoimmune diseases. Inhibition of LPAR5 reduces pain hypersensitivity and inflammatory cytokine production, making it a potential therapeutic target. Research using LPAR5 antibody supports studies in GPCR signaling, lipid biology, and inflammation.

LPAR5 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect LPA receptors. NSJ Bioreagents provides LPAR5 antibody reagents optimized for research in lipid signaling, receptor pharmacology, and immune modulation.

Structurally, Lysophosphatidic acid receptor 5 contains seven transmembrane helices and intracellular loops that couple to G proteins and scaffolding proteins. This antibody facilitates exploration of LPAR5's signaling mechanisms and receptor-mediated physiological responses.

UniProt: Q9H1C0