RPAP3 antibody (AA 84-604)

Catalog No. ABIN7876961

This Rabbit Polyclonal antibody specifically detects RPAP3 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RPAP3 antibody (AA 84-604) (ABIN7876961)

Target: RPAP3 (RNA Polymerase II Associated Protein 3 (RPAP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPAP3 Antibody

Binding Specificity: AA 84-604

Purpose: RPAP3 Antibody / RNA polymerase II-associated protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RPAP3 recombinant protein (Position: R84-E604) was used as the immunogen for the RPAP3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RPAP3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RPAP3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RPAP3

Target: RPAP3 (RNA Polymerase II Associated Protein 3 (RPAP3))

Alternative Name: RPAP3

Background: RPAP3 antibody detects RNA polymerase II-associated protein 3, encoded by the RPAP3 gene on chromosome 12q24.31. RPAP3 antibody is commonly used in transcription, chaperone, and cancer research. RPAP3 is a component of the R2TP cochaperone complex, which cooperates with HSP90 and HSP70 to stabilize and assemble large macromolecular complexes. It is involved in the maturation of RNA polymerase II, snoRNPs, PIKKs, and other critical protein assemblies.

Structurally, RPAP3 is a ~75 kDa protein with multiple tetratricopeptide repeat (TPR) domains, which mediate protein-protein interactions. These domains allow RPAP3 to bridge HSP90 and PIH1D1, central elements of the R2TP complex. Its N-terminal domain anchors it to the cochaperone machinery, while its C-terminal region facilitates substrate recognition and client protein assembly.

Functionally, RPAP3 stabilizes RNA polymerase II subunits, enabling proper transcriptional initiation and elongation. It also regulates assembly of snoRNPs, which support ribosomal RNA modification, and PIKKs, which are central to DNA damage response, nutrient sensing, and stress signaling. Researchers use RPAP3 antibody to explore protein complex maturation, transcriptional regulation, and chaperone pathways.

Clinically, RPAP3 dysregulation has been linked to cancer progression. Because it stabilizes oncogenic PIKKs such as mTOR, ATR, and ATM, RPAP3 supports tumor growth and therapy resistance. Its expression patterns may serve as biomarkers for prognosis and treatment response. NSJ Bioreagents provides RPAP3 antibody to support transcription, DNA damage response, and cancer research.

Experimentally, RPAP3 antibody is applied in western blotting to detect the ~75 kDa protein, in immunoprecipitation to study R2TP complex partners, and in immunofluorescence microscopy to visualize nuclear and cytoplasmic distribution. Chromatin immunoprecipitation using RPAP3 antibody helps examine transcriptional regulation via RNA polymerase II stabilization.

UniProt: Q9H6T3