EIF5A antibody (AA 86-154)

Catalog No. ABIN7876994

This Rabbit Polyclonal antibody specifically detects EIF5A in WB, IF, ELISA, FACS and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF5A antibody (AA 86-154) (ABIN7876994)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EIF5A Antibody

Binding Specificity: AA 86-154

Purpose: Eukaryotic translation initiation factor 5A-1 Antibody / EIF5A

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids R86-K154) was used as the immunogen for the EIF5A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Eukaryotic translation initiation factor 5A-1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Eukaryotic translation initiation factor 5A-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: Eukaryotic translation initiation factor 5A-1

Background: Eukaryotic translation initiation factor 5A-1 is a protein that in humans is encoded by the EIF5A gene. Eukaryotic initiation factor 5A (eIF5A) is an mRNA-binding protein that is involved in translation elongation and plays an important role in promoting translation of polyproline motifs. The eIF5A (eIF5A1) and eIF5A2 genes encode the two vertebrate eIF5A isoforms. While eIF5A1 is expressed constitutively in all tissues, eIF5A2 is mainly expressed in gonads. eIF5A and eIF5A2 are the only identified proteins that contain the distinctive amino acid hypusine, which is generated posttranslationally from lysine through a highly conserved polyamine metabolism pathway. eIF5A function and hypusine modification are both essential for cell proliferation, as knock down of eIF5A expression or blocking eIF5A hypusine modification suppresses cancer cell proliferation. Interestingly, eIF5A is an identified component of a tumor suppressor network of the polyamine-hypusine axis. Co-suppression of both eIF5A and adenosylmethionine decarboxylase 1 (AMD1) promotes lymphomagenesis in mice, while heterozygous deletions of the corresponding AMD1 and eIF5A genes often occur together in human lymphomas.

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation