GSG2 antibody (AA 86-798)

Catalog No. ABIN7877002

The Rabbit Polyclonal anti-GSG2 antibody (ABIN7877002) specifically detects GSG2 in WB, ELISA, ICC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GSG2 antibody (AA 86-798) (ABIN7877002)

Target: GSG2 (Germ Cell Associated 2 (Haspin) (GSG2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-GSG2 Antibody

Binding Specificity: AA 86-798

Purpose: HASPIN Antibody / Serine/threonine-protein kinase haspin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GSG2/HASPIN recombinant protein (Position: R86-K798) was used as the immunogen for the HASPIN antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HASPIN antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HASPIN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GSG2

Target: GSG2 (Germ Cell Associated 2 (Haspin) (GSG2))

Alternative Name: HASPIN

Background: The HASPIN antibody targets Serine/threonine-protein kinase haspin, a cell cycle-regulated kinase encoded by the GSG2 gene. Haspin is best known for phosphorylating histone H3 at threonine 3 (H3T3ph), a key mitotic modification that recruits the chromosomal passenger complex (CPC) to centromeres and ensures proper chromosome alignment during mitosis. The HASPIN antibody provides a critical reagent for investigating chromatin dynamics, mitotic regulation, and cell division fidelity.

Serine/threonine-protein kinase haspin is an atypical protein kinase with unique structural features distinguishing it from conventional kinase families. It remains active during mitosis and localizes to chromatin, where it marks centromeric regions to coordinate kinetochore function. The HASPIN antibody allows visualization of its distribution within dividing cells and quantification of total or active forms. This enables assessment of how Haspin integrates chromatin modification with spindle checkpoint signaling to maintain genomic stability.

Phosphorylation of histone H3 by Haspin serves as a molecular signal that recruits the Aurora B-containing CPC complex, which is essential for correcting improper microtubule-kinetochore attachments. Loss of Haspin activity disrupts chromosome segregation and leads to aneuploidy. The HASPIN antibody has therefore become a standard reagent for cell cycle and chromosome biology research, particularly in studies of mitotic kinase cascades and epigenetic checkpoint control.

In addition to its role in mitosis, Serine/threonine-protein kinase haspin has been implicated in spermatogenesis and embryonic development. Its expression in germ cells suggests functions in chromatin condensation and meiotic progression. The HASPIN antibody supports examination of these developmental contexts, providing insights into how histone phosphorylation contributes to chromatin organization beyond mitosis.

Given the importance of Haspin in maintaining genomic stability, it has emerged as a potential target for anticancer therapy. Inhibitors that block its kinase activity sensitize tumor cells to mitotic stress and disrupt proliferation. Researchers use the HASPIN antibody to evaluate target engagement and monitor cellular responses to Haspin inhibition. NSJ Bioreagents provides this antibody for reproducible performance across western blot, immunofluorescence, and immunohistochemistry assays. By enabling accurate detection of Serine/threonine-protein kinase haspin, this reagent supports the exploration of chromatin regulation, cell cycle control, and therapeutic targeting of mitotic kinases.

UniProt: Q8TF76