PC antibody (AA 88-1165)

Catalog No. ABIN7877025

The Rabbit Polyclonal anti-PC antibody is suitable to detect PC in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, IHC (p) and FACS.

Quick Overview for PC antibody (AA 88-1165) (ABIN7877025)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-PC Antibody

Binding Specificity: AA 88-1165

Purpose: Pyruvate Carboxylase Antibody / PC

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids R88-D1165) was used as the immunogen for the Pyruvate Carboxylase antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Pyruvate Carboxylase antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Pyruvate Carboxylase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvate Carboxylase

Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

UniProt: P11498