PALLD antibody (AA 89-1383)

Catalog No. ABIN7877043

The Rabbit Polyclonal anti-PALLD antibody is suitable to detect PALLD in samples from Human. It has been validated for WB and ELISA.

Quick Overview for PALLD antibody (AA 89-1383) (ABIN7877043)

Target: PALLD (Palladin, Cytoskeletal Associated Protein (PALLD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PALLD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PALLD Antibody

Binding Specificity: AA 89-1383

Purpose: Palladin Antibody / PALLD

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Palladin/PALLD recombinant protein (Position: H89-L1383) was used as the immunogen for the Palladin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Palladin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Palladin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PALLD

Target: PALLD (Palladin, Cytoskeletal Associated Protein (PALLD))

Alternative Name: Palladin

Background: Palladin antibody detects Palladin, encoded by the PALLD gene on chromosome 4q32.3. Palladin antibody is widely used in research on cytoskeletal regulation, cell adhesion, and migration. Palladin is an actin-associated protein that plays essential roles in organizing the actin cytoskeleton, stabilizing focal adhesions, and coordinating cellular motility. It is expressed in fibroblasts, smooth muscle cells, neurons, and developing tissues, where it supports cell shape and structural integrity. Palladin interacts with multiple cytoskeletal regulators, placing it at the hub of adhesion and migration networks.

Structurally, Palladin contains immunoglobulin-like (Ig-like) domains that mediate actin binding and protein-protein interactions. These domains enable Palladin to crosslink actin filaments and organize stress fibers. Palladin also interacts with focal adhesion proteins such as vinculin, alpha-actinin, and profilin. Alternative splicing generates multiple isoforms, with distinct tissue distributions and functions. These isoforms vary in size, ranging from ~90 kDa to over 200 kDa.

Functionally, Palladin regulates cell adhesion and migration by linking actin filaments to focal adhesions. It promotes stress fiber formation, lamellipodia dynamics, and directional migration. Palladin also contributes to development of tissues such as smooth muscle and nervous system by guiding cytoskeletal organization. Knockdown or mutation disrupts cell morphology and motility, confirming its essential roles. Researchers use Palladin antibody to study actin cytoskeleton biology, developmental processes, and adhesion regulation.

Clinically, Palladin has been implicated in cancer metastasis. Overexpression correlates with increased motility and invasion in pancreatic and breast cancers, while germline mutations are associated with familial pancreatic cancer. Palladin also plays roles in cardiovascular biology, where it contributes to vascular smooth muscle differentiation. Dysregulation of Palladin expression has been reported in fibrotic diseases. NSJ Bioreagents provides PALLD antibody as a reliable reagent for cytoskeletal and disease research.

Experimentally, PALLD antibody is applied in western blotting to detect isoforms of ~90i 1/2200 kDa, in immunofluorescence microscopy to study actin structures, and in immunohistochemistry to examine tissue distribution. Immunoprecipitation with Palladin antibody identifies its binding partners within adhesion complexes and cytoskeletal networks.

UniProt: Q8WX93