Ube2t antibody (AA 9-145)

Catalog No. ABIN7877065

The Rabbit Polyclonal anti-Ube2t antibody (ABIN7877065) specifically detects Ube2t in WB, ELISA, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Ube2t antibody (AA 9-145) (ABIN7877065)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ube2t antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Ube2t Antibody

Binding Specificity: AA 9-145

Purpose: UBE2T Antibody / Ubiquitin-conjugating enzyme E2 T / HSPC150

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human UBE2T recombinant protein (Position: R9-Q145) was used as the immunogen for the UBE2T antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the UBE2T antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the UBE2T antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Alternative Name: UBE2T

Background: UBE2T antibody detects Ubiquitin-conjugating enzyme E2 T, encoded by the UBE2T gene. Ubiquitin-conjugating enzyme E2 T is a member of the E2 family of enzymes that catalyze the transfer of ubiquitin to target proteins, particularly in the context of the Fanconi anemia DNA repair pathway. UBE2T antibody provides researchers with a critical reagent for studying ubiquitin signaling, DNA repair, and cancer.

Ubiquitin-conjugating enzyme E2 T functions as the cognate E2 for FANCL, the E3 ligase of the Fanconi anemia core complex. Research using UBE2T antibody has shown that it is required for monoubiquitination of FANCD2 and FANCI, a critical step in the repair of DNA interstrand crosslinks. This activity ensures faithful DNA replication and genome stability.

Studies with UBE2T antibody have revealed that loss of UBE2T function causes Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, congenital abnormalities, and cancer predisposition. Patient mutations in UBE2T impair its ubiquitin conjugating activity, preventing activation of FANCD2 and disrupting DNA repair. These findings emphasize the clinical importance of UBE2T in genome maintenance.

Beyond Fanconi anemia, dysregulation of Ubiquitin-conjugating enzyme E2 T has been associated with cancer progression. Research using UBE2T antibody has shown that overexpression promotes proliferation, survival, and resistance to DNA-damaging therapies in breast, lung, and liver cancers. This suggests potential as both a biomarker and therapeutic target.

UBE2T antibody is widely applied in western blotting, immunohistochemistry, and functional repair assays. Western blotting detects monoubiquitination of FANCD2, immunohistochemistry demonstrates tumor-associated expression, and repair assays confirm participation in crosslink repair. These applications make UBE2T antibody indispensable in DNA repair and cancer biology.

By providing validated UBE2T antibody reagents, NSJ Bioreagents supports studies into ubiquitination, DNA repair, and disease. Detection of Ubiquitin-conjugating enzyme E2 T provides researchers with insight into how ubiquitin pathways regulate genome stability and cancer progression.

UniProt: Q9NPD8

Pathways: Chromatin Binding