PARP16 antibody (AA 9-254)

Catalog No. ABIN7877069

This Rabbit Polyclonal antibody specifically detects PARP16 in FACS, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for PARP16 antibody (AA 9-254) (ABIN7877069)

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA

Product Details anti-PARP16 Antibody

Binding Specificity: AA 9-254

Purpose: PARP16 Antibody / Poly ADP-ribose polymerase 16

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PARP16 recombinant protein (Position: A9-Y254) was used as the immunogen for the PARP16 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PARP16 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PARP16 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PARP16

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Alternative Name: PARP16

Background: PARP16 antibody detects Poly(ADP-ribose) polymerase 16, a membrane-associated mono-ADP-ribosyltransferase that regulates the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress signaling. PARP16 belongs to the PARP family of enzymes involved in ADP-ribosylation, a post-translational modification that controls protein stability and signaling. The PARP16 antibody is commonly used in studies of ER stress, apoptosis, and ADP-ribosylation-dependent signaling pathways.

PARP16 is encoded by the PARP16 gene located on human chromosome 15q21.2. The protein is approximately 38 kilodaltons and uniquely localized to the ER membrane, where it functions as a tail-anchored enzyme with its catalytic domain facing the cytoplasm. Unlike canonical PARPs that catalyze poly(ADP-ribosylation), PARP16 primarily transfers single ADP-ribose moieties to substrate proteins (mono-ADP-ribosylation). This modification modulates the activity of key ER stress sensors, including PERK and IRE1-alpha, enhancing their signaling output during the UPR.

The PARP16 antibody typically detects a 38 kilodalton protein by western blot, and immunofluorescence confirms its ER localization. Functional studies demonstrate that PARP16 activity is induced upon ER stress caused by misfolded protein accumulation or chemical perturbation. Activated PARP16 promotes phosphorylation and activation of PERK and IRE1-alpha, leading to downstream transcriptional responses that restore proteostasis. Inhibition or depletion of PARP16 compromises the UPR, resulting in increased sensitivity to ER stress and enhanced apoptosis.

Beyond its role in ER stress regulation, PARP16 contributes to cancer cell survival and resistance to chemotherapeutic agents that induce proteotoxic stress. Overexpression has been reported in hepatocellular carcinoma, breast cancer, and melanoma, where it supports adaptive stress signaling. Conversely, loss of PARP16 sensitizes cells to stress-induced death, making it a potential therapeutic target. NSJ Bioreagents provides a validated PARP16 antibody optimized for immunofluorescence, immunohistochemistry and flow cytometry assays, enabling precise study of ADP-ribosylation mechanisms and ER homeostasis.

UniProt: Q8N5Y8