ETF1 antibody (AA 9-342)

Catalog No. ABIN7877076

The Mouse Monoclonal anti-ETF1 antibody is suitable to detect ETF1 in samples from Human, Mouse and Rat. It has been validated for WB, IF, FACS and IHC (p).

Quick Overview for ETF1 antibody (AA 9-342) (ABIN7877076)

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ETF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3B6

Product Details anti-ETF1 Antibody

Binding Specificity: AA 9-342

Purpose: eRF1 Antibody / EFT1

Purification: Affinity purified

Immunogen: A human recombinant partial protein (amino acids D9-K342) was used as the immunogen for the eRF1 antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the eRF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the eRF1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ETF1

Target: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Alternative Name: eRF1

Background: Eukaryotic translation termination factor 1 (eRF1), also known as TB3-1, is a protein that in humans is encoded by the ETF1 gene. It is mapped to 5q31.2. This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X.

UniProt: P62495