IDH3A antibody (AA 9-366)

Catalog No. ABIN7877079

The Rabbit Polyclonal anti-IDH3A antibody is suitable to detect IDH3A in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IF, FACS and IHC (p).

Quick Overview for IDH3A antibody (AA 9-366) (ABIN7877079)

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IDH3A Antibody

Binding Specificity: AA 9-366

Purpose: IDH3A Antibody / Isocitrate dehydrogenase [NAD] subunit alpha

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids K9-D366) was used as the immunogen for the IDH3A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IDH3A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IDH3A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IDH3A

Target: IDH3A (Isocitrate Dehydrogenase 3 (NAD+) alpha (IDH3A))

Alternative Name: IDH3A

Background: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial (IDH3?) is an enzyme that in humans is encoded by the IDH3A gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

UniProt: P50213