SHMT2 antibody (AA 9-504)

Catalog No. ABIN7877091

The Rabbit Polyclonal anti-SHMT2 antibody is suitable to detect SHMT2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ELISA, IP, ICC and FACS.

Quick Overview for SHMT2 antibody (AA 9-504) (ABIN7877091)

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SHMT2 Antibody

Binding Specificity: AA 9-504

Purpose: SHMT2 Antibody / Serine hydroxymethyltransferase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SHMT2 recombinant protein (Position: A9-H504) was used as the immunogen for the SHMT2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SHMT2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SHMT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SHMT2

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Alternative Name: SHMT2

Background: The SHMT2 antibody targets Serine hydroxymethyltransferase, mitochondrial, an enzyme encoded by the SHMT2 gene that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylenetetrahydrofolate. This reaction is central to one-carbon metabolism, providing key intermediates for nucleotide synthesis, methylation reactions, and redox regulation. Serine hydroxymethyltransferase, mitochondrial operates within the mitochondrial folate cycle and contributes to biosynthetic and energetic demands in proliferating cells. The SHMT2 antibody provides a reliable reagent for studying one-carbon metabolism, mitochondrial function, and cancer metabolism.

Serine hydroxymethyltransferase, mitochondrial forms homotetramers that utilize pyridoxal phosphate (PLP) as a cofactor for catalysis. Its activity links amino acid metabolism with folate-mediated one-carbon flux, coordinating mitochondrial and cytosolic metabolic networks. The SHMT2 antibody enables visualization of this enzyme within mitochondria, supporting studies of its spatial regulation and contribution to biosynthetic pathways under nutrient limitation or hypoxic conditions. SHMT2 expression increases in rapidly dividing cells to sustain nucleotide production and redox balance.

Aberrant activation of one-carbon metabolism, including SHMT2 upregulation, is a hallmark of cancer metabolism. Overexpression of Serine hydroxymethyltransferase, mitochondrial enhances proliferation and resistance to oxidative stress by supplying reduced folate cofactors and NADPH. The SHMT2 antibody facilitates detection of this metabolic enzyme in tumor tissues and cell lines, aiding evaluation of its role in tumorigenesis and metabolic adaptation. SHMT2 has been proposed as a potential therapeutic target, as inhibition disrupts nucleotide synthesis and redox homeostasis in cancer cells.

Beyond oncology, Serine hydroxymethyltransferase, mitochondrial contributes to neural development, mitochondrial respiration, and cellular methylation capacity. It provides one-carbon units necessary for formate generation and DNA methylation reactions that influence epigenetic regulation. The SHMT2 antibody supports research into these metabolic control points, enabling functional analysis of folate cycle dynamics in metabolic disorders, neurodegenerative diseases, and aging.

The SHMT2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing clear mitochondrial staining patterns. NSJ Bioreagents provides this antibody with validated specificity for consistent performance in biochemistry, metabolism, and cancer biology research. By enabling detailed study of Serine hydroxymethyltransferase, mitochondrial expression and activity, the SHMT2 antibody supports discovery into one-carbon metabolism and mitochondrial regulation under physiological and pathological conditions.

UniProt: P34897