FAM178A antibody (AA 90-1157)

Catalog No. ABIN7877100

The Rabbit Polyclonal anti-FAM178A antibody is suitable to detect FAM178A in samples from Human, Mouse and Rat. It has been validated for ELISA, ICC, IF and WB.

Quick Overview for FAM178A antibody (AA 90-1157) (ABIN7877100)

Target: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM178A antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Product Details anti-FAM178A Antibody

Binding Specificity: AA 90-1157

Purpose: SLF2 Antibody / SMC5-SMC6 complex localization factor protein 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SLF2 recombinant protein (Position: E90-N1157) was used as the immunogen for the SLF2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SLF2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SLF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FAM178A

Target: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Alternative Name: SLF2

Background: The SLF2 antibody targets SMC5-SMC6 complex localization factor 2, a nuclear protein encoded by the SLF2 gene that plays an essential role in DNA replication and repair. Also known as FAM178A, this protein helps recruit the SMC5/6 complex to chromatin following DNA damage, thereby promoting genome stability and replication fork recovery. SMC5-SMC6 complex localization factor 2 ensures proper DNA repair and cell cycle progression under genotoxic stress. The SLF2 antibody provides researchers with a precise tool to study DNA damage response pathways and chromosomal maintenance mechanisms.

SMC5-SMC6 complex localization factor 2 operates in coordination with its partner SLF1 to anchor the SMC5/6 complex at damaged replication forks. This localization facilitates homologous recombination repair and prevents collapse of stalled forks. The SLF2 antibody enables visualization of this critical repair factor in nuclear extracts and fixed cells, supporting detailed analysis of DNA repair kinetics and checkpoint activation. Its expression increases during replication stress and following exposure to DNA-damaging agents such as UV or ionizing radiation.

Disruption of SLF2 function compromises genomic integrity, leading to accumulation of DNA breaks, chromosomal abnormalities, and replication defects. Such genomic instability is a hallmark of cancer and aging. The SLF2 antibody aids in assessing how loss of SMC5-SMC6 recruitment affects DNA repair fidelity and cellular survival. In experimental models, depletion of SMC5-SMC6 complex localization factor 2 sensitizes cells to replication inhibitors, emphasizing its protective role during S phase.

Beyond replication stress response, SMC5-SMC6 complex localization factor 2 participates in maintaining telomere integrity and regulating chromatin compaction. The SLF2 antibody can be used to explore these broader nuclear processes, providing insight into how SMC5/6 and associated proteins orchestrate chromosomal architecture. Altered SLF2 expression has been implicated in tumorigenesis and therapy resistance, highlighting its relevance in cancer biology.

The SLF2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing strong nuclear localization consistent with its DNA repair function. NSJ Bioreagents provides this antibody as a validated reagent with reliable specificity for chromatin and genome stability research. By enabling detailed examination of SMC5-SMC6 complex localization factor 2, the SLF2 antibody supports studies into DNA repair mechanisms, replication dynamics, and cellular responses to genomic stress.

UniProt: Q8IX21