PIWIL3 antibody (AA 91-747)

Catalog No. ABIN7877146

The Rabbit Polyclonal anti-PIWIL3 antibody (ABIN7877146) specifically detects PIWIL3 in ELISA, WB and IHC. The antibody is reactive with Human samples.

Quick Overview for PIWIL3 antibody (AA 91-747) (ABIN7877146)

Target: PIWIL3 (Piwi-Like 3 (PIWIL3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIWIL3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PIWIL3 Antibody

Binding Specificity: AA 91-747

Purpose: PIWIL3 Antibody / Piwi like protein 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PIWIL3 recombinant protein (Position: Q91-K747) was used as the immunogen for the PIWIL3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PIWIL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PIWIL3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PIWIL3

Target: PIWIL3 (Piwi-Like 3 (PIWIL3))

Alternative Name: PIWIL3

Background: PIWIL3 antibody detects Piwi like protein 3, encoded by the PIWIL3 gene. Piwi like protein 3 belongs to the Argonaute family of proteins and plays a critical role in germline development, small RNA regulation, and maintenance of genomic integrity. PIWIL3 antibody provides researchers with a specialized reagent for studying piRNA pathways, transposon silencing, and fertility biology.

Piwi like protein 3 is predominantly expressed in oocytes and early embryos, where it participates in RNA silencing through interaction with piRNAs. Research using PIWIL3 antibody has shown that it forms ribonucleoprotein complexes that suppress transposable elements, protecting genome stability during germline development. Its activity helps ensure faithful transmission of genetic material to the next generation.

Studies with PIWIL3 antibody have revealed that this protein contributes to oocyte maturation and embryonic development. It works in conjunction with other PIWI family proteins to organize RNA regulatory pathways essential for fertility. Loss of PIWIL3 activity disrupts piRNA pathways, leading to increased transposon mobilization, DNA damage, and impaired germline development.

Dysregulation of Piwi like protein 3 has been associated with infertility and reproductive failure. Research using PIWIL3 antibody has shown that reduced expression contributes to compromised oocyte quality and embryonic arrest, while altered expression patterns may also play roles in ovarian cancer. These findings highlight its importance in both developmental and disease contexts.

PIWIL3 antibody is widely used in immunohistochemistry, western blotting, and immunofluorescence. Immunohistochemistry identifies localization in ovarian tissue, western blotting quantifies expression during germ cell development, and immunofluorescence highlights piRNA granule distribution in oocytes. These applications make PIWIL3 antibody indispensable for reproductive biology research.

By supplying validated PIWIL3 antibody reagents, NSJ Bioreagents supports studies into small RNA pathways, transposon control, and germline development. Detection of Piwi like protein 3 provides researchers with insight into how piRNA machinery regulates genome stability and reproductive success.

UniProt: Q7Z3Z3