SYNM antibody (AA 93-1554)

Catalog No. ABIN7877178

The Rabbit Polyclonal anti-SYNM antibody is suitable to detect SYNM in samples from Human and Mouse. It has been validated for ELISA, WB and FACS.

Quick Overview for SYNM antibody (AA 93-1554) (ABIN7877178)

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYNM antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SYNM Antibody

Binding Specificity: AA 93-1554

Purpose: SYNM Antibody / Synemin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SYNM recombinant protein (Position: R93-D1554) was used as the immunogen for the SYNM antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SYNM antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SYNM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SYNM

Target: SYNM (Synemin, Intermediate Filament Protein (SYNM))

Alternative Name: SYNM

Background: The SYNM antibody targets Synemin, a large intermediate filament-associated protein encoded by the SYNM gene. Synemin belongs to the intermediate filament (IF) protein family and serves as a cytoskeletal crosslinker that connects desmin and vimentin filaments to cellular structures. Through these interactions, Synemin helps maintain cellular architecture, mechanical stability, and signaling dynamics. The SYNM antibody allows precise detection of Synemin to study cytoskeletal organization and mechanotransduction in muscle and non-muscle cells.

Synemin exists in multiple isoforms, including alpha and beta forms, which differ in size and tissue distribution. The alpha isoform is primarily expressed in striated muscle, while the beta isoform occurs in smooth muscle and non-muscle tissues such as astrocytes and hepatocytes. Both isoforms contain a conserved intermediate filamenti 1/2binding domain that mediates filament anchoring and a unique C-terminal tail that interacts with cytoskeletal and signaling proteins. The SYNM antibody detects these isoforms, enabling evaluation of Synemin expression across tissues and developmental stages.

Functionally, Synemin acts as a structural scaffold linking intermediate filaments to focal adhesions, costameres, and the plasma membrane. It binds to alpha-actinin, dystrophin, and protein kinase A regulatory subunits, integrating cytoskeletal mechanics with signal transduction. Loss or mutation of Synemin disrupts cell shape, elasticity, and contractility, emphasizing its role in maintaining structural integrity. Researchers employ the SYNM antibody to characterize these cytoskeletal connections and assess their impact on tissue strength and cellular signaling.

Synemin expression is prominent in cardiac and skeletal muscle, where it contributes to sarcomere alignment and force transmission. Dysregulation of Synemin may contribute to muscular dystrophies, cardiomyopathies, and other myopathic conditions. The SYNM antibody supports these investigations by providing a consistent and specific reagent for identifying Synemin in muscle biopsies, cultured myocytes, and tissue sections. Beyond muscle, Synemin has been implicated in glial and epithelial cell structure, suggesting roles in neurobiology and cancer progression.

Experimental applications of the SYNM antibody include western blotting, immunofluorescence, and immunohistochemistry. These assays reveal Synemin's filamentous distribution and dynamic rearrangement during cell migration or mechanical stress. NSJ Bioreagents supplies the SYNM antibody to facilitate ongoing research into cytoskeletal organization, signal transduction, and mechanobiology. Its reproducible specificity ensures reliable detection across experimental platforms, enabling detailed study of how Synemin contributes to cellular resilience, structure, and disease processes.

UniProt: O15061