PRPF18 antibody (AA 93-342)

Catalog No. ABIN7877181

The Rabbit Polyclonal anti-PRPF18 antibody is suitable to detect PRPF18 in samples from Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for PRPF18 antibody (AA 93-342) (ABIN7877181)

Target: PRPF18 (PRP18 Pre-mRNA Processing Factor 18 (PRPF18))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPF18 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRPF18 Antibody

Binding Specificity: AA 93-342

Purpose: Zebrafish Prpf18 Antibody / Pre-mRNA-splicing factor 18

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Prpf18 recombinant protein (amino acids E93-L342) was used as the immunogen for the Zebrafish Prpf18 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Prpf18 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Prpf18 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PRPF18

Target: PRPF18 (PRP18 Pre-mRNA Processing Factor 18 (PRPF18))

Alternative Name: Prpf18

Background: Prpf18 (Pre-mRNA processing factor 18) is a key component of the spliceosome, the large ribonucleoprotein complex responsible for the removal of introns from precursor messenger RNA during gene expression. Prpf18 plays an essential role in the second catalytic step of splicing, contributing to the proper assembly and function of the spliceosomal complex and ensuring the accuracy of mRNA processing.

In zebrafish, Prpf18 is an ortholog of the human PRPF18 gene. The zebrafish and human proteins are highly conserved, particularly within domains essential for spliceosomal interactions and catalytic activity. This conservation suggests functional equivalency between species, making zebrafish a relevant model for studying pre-mRNA splicing and its regulation in development and disease.

Zebrafish Prpf18 may have isoforms arising from alternative splicing events, although isoform-specific functions are still under investigation. The presence of multiple isoforms may allow differential regulation of splicing in a tissue-specific or developmental stage-specific manner.

During zebrafish embryogenesis, Prpf18 is broadly expressed, with notable enrichment in rapidly dividing and transcriptionally active tissues such as the neural tube and developing somites. Its expression is crucial for early development, as disruption of splicing machinery can lead to widespread defects in gene regulation, cell cycle control, and differentiation.

Given its central role in RNA splicing, zebrafish Prpf18 is important for studies related to gene expression regulation, developmental biology, and spliceosome-associated disorders. It also serves as a useful model for understanding human genetic diseases caused by splicing defects, including certain neurodegenerative and retinal disorders.

UniProt: Q6GMH0