GJA4 antibody (AA 95-148)

Catalog No. ABIN7877214

The Rabbit Polyclonal anti-GJA4 antibody is suitable to detect GJA4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for GJA4 antibody (AA 95-148) (ABIN7877214)

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GJA4 Antibody

Binding Specificity: AA 95-148

Purpose: GJA4 Antibody / Gap junction alpha 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GJA4 recombinant protein (Position: H95-R148) was used as the immunogen for the GJA4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GJA4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GJA4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GJA4

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Alternative Name: GJA4

Background: GJA4 antibody detects Gap junction alpha-4 protein, also known as Connexin 37, a transmembrane protein forming intercellular channels that mediate direct communication between adjacent cells. GJA4 enables diffusion of ions, metabolites, and signaling molecules, maintaining vascular tone and tissue homeostasis. The GJA4 antibody is widely used in cardiovascular, reproductive, and cellular communication studies to explore gap junction biology and endothelial signaling.

GJA4 is encoded by the GJA4 gene on human chromosome 1p35.1. The protein is approximately 333 amino acids long and contains four transmembrane helices, two extracellular loops, and cytoplasmic N- and C-terminal domains. GJA4 assembles into hexameric connexons that dock with connexons of neighboring cells, forming gap junction channels that permit electrical and chemical coupling.

The GJA4 antibody detects a 37 kilodalton band by western blot and shows punctate membrane staining consistent with gap junction plaques under immunofluorescence. GJA4 is abundantly expressed in vascular endothelial and smooth muscle cells, where it mediates synchronized contraction and vascular tone regulation. In ovarian follicles and the placenta, GJA4 facilitates cell communication critical for follicular development and embryonic exchange.

Mutations in GJA4 disrupt gap junction communication, contributing to vascular dysfunction and reproductive disorders. Altered GJA4 expression has been observed in atherosclerosis, hypertension, and tumor angiogenesis. Its expression pattern varies with hormonal and mechanical stimuli, reflecting its responsiveness to physiological cues controlling vascular integrity.

GJA4 channels are regulated by phosphorylation, pH , and redox state, allowing dynamic modulation of intercellular connectivity. Because gap junctions coordinate tissue-level responses, GJA4 represents a vital component of multicellular signaling networks. NSJ Bioreagents provides a validated GJA4 antibody optimized for its applications, supporting detailed studies of cell coupling, signal propagation, and endothelial homeostasis.

UniProt: P35212

Pathways: Cell-Cell Junction Organization