ENTPD5 antibody (AA 95-428)

Catalog No. ABIN7877223

The Rabbit Polyclonal anti-ENTPD5 antibody is suitable to detect ENTPD5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and FACS.

Quick Overview for ENTPD5 antibody (AA 95-428) (ABIN7877223)

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENTPD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-ENTPD5 Antibody

Binding Specificity: AA 95-428

Purpose: ENTPD5 Antibody / Ectonucleoside triphosphate diphosphohydrolase 5

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ENTPD5 recombinant protein (Position: D95-H428) was used as the immunogen for the ENTPD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ENTPD5 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ENTPD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ENTPD5

Target: ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))

Alternative Name: ENTPD5

Background: ENTPD5 antibody detects Ectonucleoside triphosphate diphosphohydrolase 5, an endoplasmic reticulum (ER)-localized enzyme that regulates protein N-glycosylation and energy metabolism. The UniProt recommended name is Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5). Unlike other ENTPD family members that function extracellularly, ENTPD5 acts within the ER lumen, promoting protein folding and glycoprotein maturation through its unique enzymatic cycle.

Functionally, ENTPD5 antibody identifies a 428-amino-acid enzyme that hydrolyzes UDP and GDP to UMP and GMP, coupling its activity to the UDP-glucose pyrophosphorylase reaction that regenerates UDP-glucose. This cycle supports proper glycoprotein processing by maintaining a continuous supply of glycosylation precursors within the ER. ENTPD5 activity enhances protein folding capacity and assists in the maturation of membrane receptors and secretory proteins.

The ENTPD5 gene is located on chromosome 14q24.1 and is expressed in metabolically active tissues such as liver, pancreas, and muscle. Its expression is transcriptionally upregulated by AKT signaling and MYC oncogene activation, linking metabolic reprogramming to cancer cell growth. ENTPD5 contributes to the Warburg effect by coupling glycosylation demand to glycolytic flux in proliferating cells.

Pathologically, overexpression of ENTPD5 promotes tumor progression by increasing glycoprotein folding and surface receptor stability, enhancing proliferative signaling. Conversely, loss of ENTPD5 impairs growth factor receptor maturation and induces ER stress. Elevated ENTPD5 expression has been observed in prostate, colon, and liver cancers. Research using ENTPD5 antibody supports studies in cancer metabolism, glycoprotein folding, and ER homeostasis.

ENTPD5 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect ER-localized glycoprotein processing enzymes. NSJ Bioreagents provides ENTPD5 antibody reagents optimized for studies in protein maturation, metabolic regulation, and oncogenic signaling.

Structurally, Ectonucleoside triphosphate diphosphohydrolase 5 contains an apyrase conserved region (ACR) and calcium-binding motifs essential for nucleotide hydrolysis. It forms dimers in the ER lumen and associates with chaperones such as calnexin and calreticulin. This antibody enables exploration of ENTPD5's role in glycoprotein biosynthesis, ER stress adaptation, and cancer metabolism.

UniProt: O75356